Intravenous administration of human bone marrow stromal cells induces angiogenesis in the ischemic boundary zone after stroke in rats
Intravenous administration of human bone marrow stromal cells induces angiogenesis in the ischemic boundary zone after stroke in rats
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DOI：
10.1161/01.res.0000063425.51108.8d
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发表时间：
2003-04-04
影响因子：
20.1
通讯作者：
Chopp, M
中科院分区：
文献类型：
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作者：
Chen, JL;Zhang, ZG;Chopp, M
我们检验了静脉输注人骨髓基质细胞（hMSCs）促进血管内皮生长因子（VEGF）分泌、VEGF受体2（VEGFR2）表达和卒中后缺血边界区（IBZ）血管生成的假设。在大脑中动脉闭塞（MCAo）后24小时将hMSC（1 × 106）静脉内注射到大鼠中。采用激光扫描共聚焦显微镜（LSCM）、免疫组织化学和ELISA法分别检测宿主脑中血管生成和人和大鼠VEGF的水平。此外，使用小鼠脑源性内皮细胞（MBDEC）测量毛细血管样管形成。形态学和三维图像分析显示，
We tested the hypothesis that intravenous infusion of human bone marrow stromal cells (hMSCs) promotes vascular endothelial growth factor (VEGF) secretion, VEGF receptor 2 (VEGFR2) expression and angiogenesis in the ischemic boundary zone (IBZ) after stroke. hMSCs (1 x 10(6)) were intravenously injected into rats 24 hours after middle cerebral artery occlusion (MCAo). Laser scanning confocal microscopy (LSCM), immunohistochemistry and ELISA were performed to assay angiogenesis and levels of human and rat VEGF in the host brain, respectively. In addition, capillary-like tube formation was measured using mouse brain-derived endothelial cells (MBDECs). Morphological and three dimensional image analyses revealed significant (P