Development and verification of wheat germplasm containing both Sr2 and Fhb1
Development and verification of wheat germplasm containing both Sr2 and Fhb1
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DOI：
10.1007/s11032-016-0502-y
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发表时间：
2016-06
影响因子：
3.1
通讯作者：
Xiaofei Zhang;M. Rouse;I. C. Nava;Yue Jin;J. Anderson
中科院分区：
文献类型：
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作者：
Xiaofei Zhang;M. Rouse;I. C. Nava;Yue Jin;J. Anderson
赤霉病（FHB）和茎锈病是世界范围内流行的两种常见小麦毁灭性真菌病害。Fhb1是众所周知的有效对抗FHB的主要数量性状基因座，Sr2是最广泛表征和应用的小麦茎锈病成株抗性基因。这些基因在 3B 号染色体上以排斥相相连，相距约 3 cM。为了获得同时含有Fhb1和Sr2基因的小麦种质，我们使用紧密连锁的DNA标记Xgwm533和csSr2（Sr2）和UMN10以及新设计的Fhb1标记UMN10v2开发和筛选了两个F2群体。 1949株F2植物中，有43株被鉴定为Fhb1和Sr2处于耦合期。通过春化基因标记筛选，获得Fhb1和Sr2纯合的F3春小麦品系59个。所有这些品系在田间表现出的 FHB 严重程度均显着低于易感品系 (P< 0.01)，并且 FHB 严重程度与田间耐药品系相似 (P> 0.61)。这些品系在温室试验中作为幼苗很敏感，但作为成年植物对茎锈病苗圃中的 QTHJC 竞赛具有抗性。田间抗病性评估证实，所有这些重组品系均对FHB和茎锈病表现出中度或高水平的抗性。该种质将有利于抗 FHB 和茎锈病的育种工作，并且获得重组体的程序适用于其他小麦育种计划。
Fusariumhead blight (FHB) and stem rust are two devastating fungal diseases of common wheat prevalent worldwide.Fhb1is a well-known major quantitative trait locus effective against FHB, andSr2is the most widely characterized and deployed wheat stem rust adult plant resistance gene. These genes are linked in repulsion phase, approximately 3 cM apart, on chromosome 3B. To obtain wheat germplasm containing bothFhb1andSr2genes, we developed and screened two F2populations using tightly linked DNA markers, Xgwm533 and csSr2 forSr2, and UMN10, and a newly designed marker UMN10v2forFhb1. Among 1949 F2plants, 43 were identified withFhb1andSr2in coupling phase. After screened with vernalization gene markers, 59 F3spring wheat lines with homozygousFhb1andSr2were obtained. All these lines showed significantly lower FHB severity than susceptible checks (P< 0.01) and similar FHB severity to the resistant checks (P> 0.61) in the field. And these lines were susceptible as seedlings in greenhouse assays, but resistant as adult plants to race QTHJC in the stem rust nursery. Field disease resistance evaluation verified that all these recombinant lines showed moderate or high levels of resistance to both FHB and stem rust. This germplasm would benefit efforts to breed for resistance to FHB and stem rust, and the procedure to obtain recombinants is adaptable to other wheat breeding programs.