INHIBITION OF ARGINASE BY N-G-HYDROXY-L-ARGININE IN ALVEOLAR MACROPHAGES - IMPLICATIONS FOR THE UTILIZATION OF L-ARGININE FOR NITRIC-OXIDE SYNTHESIS
INHIBITION OF ARGINASE BY N-G-HYDROXY-L-ARGININE IN ALVEOLAR MACROPHAGES - IMPLICATIONS FOR THE UTILIZATION OF L-ARGININE FOR NITRIC-OXIDE SYNTHESIS
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DOI：
10.1016/0014-5793(95)00039-c
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发表时间：
1995-02-13
期刊：
影响因子：
3.5
通讯作者：
RACKE, K
中科院分区：
文献类型：
--
作者：
HECKER, M;NEMATOLLAHI, H;RACKE, K
在兔和大鼠肺泡巨噬细胞中，一氧化氮合酶中间体N-G-羟基-L-精氨酸(HOArg)是一种精氨酸酶抑制剂，外源性HOArg强烈抑制这些细胞的精氨酸酶活性(IC(50)大于或等于15mU M)，并减弱兔肺泡巨噬细胞的L-[H-3]精氨酸转运(IC(50)大于或等于500mU M)。此外，在细菌脂多糖作用18h的大鼠肺泡巨噬细胞的条件培养液中检测到高达37mM的HOArg，而在裂解液中未检测到HOArg。因此，HOArg可能是这些细胞中一种有效的内源性精氨酸酶抑制剂，从而增加了L-精氨酸对NO生物合成的利用率。
The hypothesis was investigated that the nitric oxide (NO) synthase intermediate, N-G-hydroxy-L-arginine (HOArg), is an arginase inhibitor in rabbit or rat alveolar macrophages, Exogenously applied HOArg strongly inhibited the arginase activity present in these cells (IC(50)greater than or equal to 15 mu M), and attenuated L-[H-3]arginine transport (IC50 greater than or equal to 500 mu M) in rabbit alveolar macrophages. Moreover, up to 37 mu M HOArg were detected in the conditioned medium, but not in the lysate, of rat alveolar macrophages exposed to bacterial lipopolysaccharide for 18 h, HOArg may thus be a potent endogenous arginase inhibitor in these cells which increases the availability of L-arginine for NO biosynthesis.