Cross-species amplification of microsatellite loci within the dioecious, polyploid genus Actinidia (Actinidiaceae)
Cross-species amplification of microsatellite loci within the dioecious, polyploid genus Actinidia (Actinidiaceae)
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DOI：
10.1007/s00122-005-0117-x
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发表时间：
2005-12-01
影响因子：
5.4
通讯作者：
De Silva, HN
中科院分区：
文献类型：
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作者：
Fraser, LG;McNeilage, MA;De Silva, HN
猕猴桃雌雄异株猕猴桃属（Actinidia，猕猴桃）的微卫星标记跨种转移可作为一种高效、省时的性状转移技术，用于葡萄和果实的育种改良。我们评估了20个est衍生的微卫星标记的跨种扩增，这些标记在中国猕猴桃作图科中具有充分的信息。我们测试了所有20个标记在21个种的120个基因型上，5个有变种和/或染色体小种。这26个分类群包括16个二倍体、7个四倍体、2个六倍体和1个八倍体，代表了该属的所有4个分类区段。所有20个标记均表现出一定程度的跨种扩增。扩增最成功的标记片段只在中国沙蚕和美味沙蚕中扩增最不成功。其中一种青花蒿（A. glaucophylla）除2个标记外，其余标记均扩增失败。多态性信息含量（PIC）值较高，17个标记中有14个标记的记录值在0.90及以上。序列数据表明，所有扩增产物均存在微卫星。序列同源性在微卫星上小于5′，并向产生标记的EST翻译区的起始密码子方向增加。结果表明，猕猴桃est衍生的微卫星标记具有较高的跨种扩增和多态性，可作为猕猴桃育种的有用标记。
Microsatellite marker transfer across species in the dioecious genus Actinidia (kiwifruit) could offer an efficient and time-effective technique for use during trait transfer for vine and fruit improvement in breeding programmes. We evaluated the cross-species amplification of 20 EST-derived microsatellite markers that were fully informative in an Actinidia chinensis mapping family. We tested all 20 markers on 120 genotypes belonging to 21 species, 5 with varieties and/or chromosome races. These 26 taxa included 16 diploids, 7 tetraploids, 2 hexaploids and 1 octaploid, and represented all four taxonomic sections in the genus. All 20 markers showed some level of cross-species amplification. The most successful marker amplified in all genotypes from all species from all sections of the genus, the least successful amplified fragments only in A. chinensis and A. deliciosa. One species, A. glaucophylla, failed to amplify with all but 2 markers. PIC (Polymorphism information content) values were high, with 14 of 17 markers recording values of 0.90 and above. Sequence data demonstrated the presence of the microsatellite in all the amplified products. Sequence homology was less 5' of the microsatellite and increased toward the start codon of the translated region of the EST from which the marker was derived. The data confirm that EST-derived microsatellite markers from Actinidia species show cross-species amplification with high levels of polymorphism which could make them useful markers in breeding programmes.