Transforming activity of purinergic receptor P2Y, G-protein coupled, 2 revealed by retroviral expression screening
Transforming activity of purinergic receptor P2Y, G-protein coupled, 2 revealed by retroviral expression screening
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DOI：
10.1016/j.bbrc.2007.03.048
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发表时间：
2007-05-11
影响因子：
3.1
通讯作者：
Mano, Hiroyuki
中科院分区：
文献类型：
--
作者：
Hatanaka, Hisashi;Takada, Shuji;Mano, Hiroyuki
结直肠癌（CRC）是人类癌症死亡的主要原因之一。为了鉴定CRC中新的促癌基因，我们从CRC细胞系RKO中构建了逆转录病毒cDNA表达文库，并将其用于小鼠3T3成纤维细胞的病灶形成试验，从而鉴定出42个独立的cDNA。其中一个cDNA编码嘌呤能受体P2Y，G蛋白偶联，2（P2RY2）。P2RY2的致癌潜力在体外用病灶形成测定以及软琼脂生长测定证实，并且在体内用裸鼠中的致瘤性测定证实。虽然我们的P2RY2 cDNA编码的蛋白质与两个氨基酸取代相比，报告的一个，我们已经证实，野生型P2RY2具有很强的转化潜力，以及。这些结果表明P2RY2在人类癌症的致癌作用中具有意想不到的作用。(c)2007年爱思唯尔公司All rights reserved.
Colorectal cancer (CRC) is one of the leading causes of cancer death in humans. In order to identify novel cancer-promoting genes in CRC, we here constructed a retroviral cDNA expression library from a CRC cell line RKO, and used it for a focus formation assay with mouse 3T3 fibroblasts, leading to the identification of 42 independent cDNAs. One of such cDNAs turned out to encode purinergic receptor P2Y, G-protein coupled, 2 (P2RY2). The oncogenic potential of P2RY2 was confirmed in vitro with the focus formation assay as well as soft agar-growth assay, and also in vivo with a tumorigenicity assay in nude mice. While our P2RY2 cDNA encodes a protein with two amino-acid substitutions compared to the reported one, we have confirmed that the wild-type P2RY2 has a strong transforming potential as well. These results indicate an unexpected role of P2RY2 in the carcinogenesis of human cancers. (c) 2007 Elsevier Inc. All rights reserved.