The transcriptional factor Osterix directly interacts with RNA helicase A
The transcriptional factor Osterix directly interacts with RNA helicase A
复制标题
DOI：
10.1016/j.bbrc.2007.01.150
复制
发表时间：
2007-04-06
影响因子：
3.1
通讯作者：
Haneji, Tatsuji
中科院分区：
文献类型：
--
作者：
Amorim, Bruna Rabelo;Okamura, Hirohiko;Haneji, Tatsuji
Osterix是一种成骨细胞特异性转录因子，是骨形成和成骨细胞分化所必需的。在这里，我们利用基质辅助激光解吸电离飞行时间质谱鉴定了新的Osterix相互作用因子。其中，RNA解旋酶A被确定与Osterix相互作用。采用免疫沉淀法测定Osterix与RNA解旋酶A的相互作用。Western分析证实了Osterix与RNA解旋酶A之间的关联。免疫细胞化学分析也表明Osterix与RNA解旋酶A在HEK 293细胞中共定位。我们的数据表明RNA解旋酶A可能是Osterix调控的一个组成部分。(c) 2007爱思唯尔公司版权所有。
Osterix is an osteoblast-specific transcriptional factor, required for bone formation and osteoblast differentiation. Here, we identified new Osterix interacting factors by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Among the candidates, RNA helicase A was identified to interact with Osterix. To determine the interaction of Osterix with RNA helicase A, immunoprecipitation assay was performed. Western analysis confirmed the association between Osterix and RNA helicase A. Immunocytochemical analysis also showed that Osterix and RNA helicase A were co-localized in HEK 293 cells. Our data suggest that RNA helicase A might be a component of Osterix regulation. (c) 2007 Elsevier Inc. All rights reserved.