EFFECT OF DIADENOSINE POLYPHOSPHATES ON CATECHOLAMINE SECRETION FROM ISOLATED CHROMAFFIN CELLS
EFFECT OF DIADENOSINE POLYPHOSPHATES ON CATECHOLAMINE SECRETION FROM ISOLATED CHROMAFFIN CELLS
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DOI：
10.1111/j.1476-5381.1990.tb15809.x
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发表时间：
1990-06-01
影响因子：
7.3
通讯作者：
GONZALEZ, MP
中科院分区：
文献类型：
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作者：
CASTRO, E;TORRES, M;GONZALEZ, MP
1.研究了几种二腺苷多磷酸(AP3A、AP4A和AP5A)对基础和高K~+-尼古丁诱发的儿茶酚胺(CA)释放的影响。三种二腺苷多磷酸都微弱地促进了基础CA的分泌，但显著增加。这一增强约占2µm尼古丁引起的反应的10%。2.多聚磷酸二腺苷诱导的分泌反应对细胞外钙离子有绝对的需求。3.相反，这些化合物对尼古丁诱发的释放有抑制作用。这种反应是浓度依赖性的，EC50值为3.2+-。0.4µm，4.0.+-。1.6µm和19.3.+-。分别用于AP3A、AP4A和AP5A的4.0微米。用来刺激分泌的尼古丁浓度越低，这些化合物的抑制力就越高。4.AP3A和AP5A可进一步促进富K+溶液诱导的CA分泌，而AP4A则抑制其分泌。讨论了这些双重作用可能的生理作用。
1. The action of several diadenosine polyphosphates (AP3A, AP4A and AP5A) on basal, and on nicotine- and high K+-evoked, catecholamine (CA) release has been investigated. Each of three diadenosine polyphosphates weakly but significantly increased basal CA secretion. This enhancement represented about 10% of the response evoked by 2 .mu.M nicotine. 2. The evoked secretory response to diadenosine polyphosphates had an absolute requirement for extracellular Ca2+. 3. In contrast, these compounds had an inhibitory action on nicotine-evoked release. This response was concentration-dependent, EC50 values being 3.2 .+-. 0.4 .mu.M, 4.0 .+-. 1.6 .mu.m and 19.3 .+-. 4.0 .mu.m for AP3A, AP4A, and AP5A, respectively. The lower the concentration of nicotine used to evoke secretion, the higher the inhibitory power of these compounds. 4. The CA secretion evoked by K+-rich solutions was further enhanced by AP3A and AP5A, whereas AP4A inhibited it. The possible physiological role of these dual actions is discussed.