First-generation neuronal precursors in the crayfish brain are not self-renewing.
First-generation neuronal precursors in the crayfish brain are not self-renewing.
复制标题
小龙虾脑中的第一代神经元前体并非自我更新。
DOI：
10.1016/j.ijdevneu.2012.11.010
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发表时间：
2013-11
期刊：
影响因子：
--
通讯作者：
Beltz BS
中科院分区：
文献类型：
--
作者：
Benton JL;Chaves da Silva PG;Sandeman DC;Beltz BS
克氏原螯虾（Procambarus clarkii）的成体神经元是第一代前体细胞（功能类似于脊椎动物的神经干细胞）的后代，位于大脑腹侧表面的神经源性生态位。这些前体细胞的子细胞沿着双极小生境细胞的过程迁移到嗅觉中间神经元体细胞所在的细胞簇的增殖区。在这里，它们再次分裂，产生分化为嗅觉局部神经元和投射神经元的后代。这条神经元装配线的特点，以及当大脑被分离、灌注或维持在器官型培养中时，它继续发挥作用的事实，为探索导致成人大脑中新神经元产生的细胞和分子事件的序列提供了在其他生物体中无法获得的机会。此外，我们已经确定，第一代前体细胞不是自我更新的种群，然而，生态位不会随着动物的生长和衰老而耗尽。因此，我们得出结论，这个生态位不是一个封闭的系统，必须有一个外部的神经干细胞来源。基于体外研究表明，从血淋巴中提取的细胞被生态位吸引，以及生态位与脉管系统之间的密切关系，我们假设造血系统可能是这些细胞的来源。
Adult-born neurons in crayfish (Procambarus clarkii) are the progeny of 1st-generation precursor cells (functionally analogous to neuronal stem cells in vertebrates) that are located in a neurogenic niche on the ventral surface of the brain. The daughters of these precursor cells migrate along the processes of bipolar niche cells to proliferation zones in the cell clusters where the somata of the olfactory interneurons reside. Here they divide again, producing offspring that differentiate into olfactory local and projection neurons. The features of this neuronal assembly line, and the fact that it continues to function when the brain is isolated and perfused or maintained in organotypic culture, provide opportunities unavailable in other organisms to explore the sequence of cellular and molecular events leading to the production of new neurons in adult brains. Further, we have determined that the 1st-generation precursor cells are not a self-renewing population, and that the niche is, nevertheless, not depleted as the animals grow and age. We conclude, therefore, that the niche is not a closed system and that there must be an extrinsic source of neuronal stem cells. Based on in vitro studies demonstrating that cells extracted from the hemolymph are attracted to the niche, as well as the intimate relationship between the niche and vasculature, we hypothesize that the hematopoietic system is a likely source of these cells.
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影响因子：
4.5
作者：
Deng, Y-B;Liu, X-G;Zhou, G-Q
通讯作者：
Zhou, G-Q
DOI：
10.1073/pnas.0336479100
发表时间：
2003-02-04
影响因子：
11.1
作者：
Mezey, É;Key, S;Crain, B
通讯作者：
Crain, B
影响因子：
168.9
作者：
Cogle, CR;Yachnis, AT;Scott, EW
通讯作者：
Scott, EW
影响因子：
3.4
作者：
Beltz, Barbara S.;Zhang, Yi;Sandeman, David C.
通讯作者：
Sandeman, David C.
影响因子：
5.3
作者：
Crigler, L;Robey, RC;Phinney, DG
通讯作者：
Phinney, DG