Cytochrome P450 family member CYP96B5 hydroxylates alkanes to primary alcohols and is involved in rice leaf cuticular wax synthesis
Cytochrome P450 family member CYP96B5 hydroxylates alkanes to primary alcohols and is involved in rice leaf cuticular wax synthesis
复制标题
细胞色素 P450 家族成员 CYP96B5 将烷烃羟基化为伯醇，并参与稻叶角质层蜡的合成
DOI：
10.1111/nph.16267
复制
发表时间：
2020
期刊：
影响因子：
9.4
通讯作者：
Le Qing Qu
中科院分区：
文献类型：
--
作者：
Du Zhang;Huifang Yang;Xiaochen Wang;Yijian Qiu;Lihong Tian;Xiaoquan Qi;Le Qing Qu
奇数伯醇是植物角质蜡的组成成分，但它们的生物合成尚不清楚。我们利用基于图谱的克隆策略分离了一个米蜡晶体稀疏叶5(WSL5)基因。WSL5在水稻中的过表达和敲除、在拟南芥中的异源表达和在烟草叶片中的瞬时表达都说明了WSL5的功能，预测WSL5编码一个细胞色素P450家族成员CYP96B5。Wsl5突变体的角质膜表面缺乏晶状小片，且角质膜厚度大于野生型。Wsl5突变体对干旱胁迫的耐受性较强。在WSL5突变体和WSL5基因敲除转基因植株中，C23-C33烷烃的负载量显著增加，而C29伯醇的负载量显著减少。WSL5基因的过表达增加了水稻叶片中C29的伯醇含量，降低了烷烃含量。WSL5的异源表达增加了拟南芥茎蜡中C29的伯醇含量，降低了烷烃、仲醇和酮的含量。WSL5在烟草叶片中的瞬时表达也增加了C29伯醇的产生。WSL5催化烷烃的末端羟基化，产生奇数的伯醇，并参与水稻叶片上表皮蜡晶的形成，影响干旱敏感性。
Odd‐numbered primary alcohols are components of plant cuticular wax, but their biosynthesis remains unknown.We isolated a ricewax crystal‐sparse leaf 5(WSL5) gene using a map‐based cloning strategy. The function ofWSL5was illustrated by overexpression and knockout in rice, heterologous expression in Arabidopsis and transient expression in tobacco leaves.WSL5is predicted to encode a cytochrome P450 family member CYP96B5. Thewsl5mutant lacked crystalloid platelets on the surface of cuticle membrane, and its cuticle membrane was thicker than that of the wild‐type. Thewsl5mutant is more tolerant to drought stress. The load of C23–C33alkanes increased, whereas the C29primary alcohol reduced significantly inwsl5mutant andWSL5knockout transgenic plants. Overexpression ofWSL5increased the C29primary alcohol and decreased alkanes in rice leaves. Heterologous expression ofWSL5increased the C29primary alcohol and decreased alkanes, secondary alcohol, and ketone in Arabidopsis stem wax. Transient expression ofWSL5in tobacco leaves also increased the production C29primary alcohol.WSL5 catalyzes the terminal hydroxylation of alkanes, yielding odd‐numbered primary alcohols, and is involved in the formation of epidermal wax crystals on rice leaf, affecting drought sensitivity.