Effect Of Five Proteases Including Alcalase, Flavourzyme, Papain, Proteinase K And Trypsin On Antioxidative Activities Of Casein Hydrolysate From Goat Milk
Effect Of Five Proteases Including Alcalase, Flavourzyme, Papain, Proteinase K And Trypsin On Antioxidative Activities Of Casein Hydrolysate From Goat Milk
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碱性蛋白酶、风味酶、木瓜蛋白酶、蛋白酶K和胰蛋白酶5种蛋白酶对羊奶酪蛋白水解物抗氧化活性的影响
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Hong Li
中科院分区：
文献类型：
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作者：
G. Shu;Qian Zhang;He Chen;Hongchang Wan;Hong Li
摘要氧化与人类疾病的发病机制有关。摄入足够的抗氧化剂活性的食物，可以降低自由基水平，防止脂质过氧化，帮助身体抵御疾病。以羊奶中的酪蛋白为原料，采用碱性酶、风味酶、木瓜蛋白酶、K酶和胰酶等五种商品酶对羊奶中的酪蛋白进行酶解。通过测定水解度、清除DPPH自由基活性、金属螯合活性和清除超氧阴离子自由基活性来评价酪蛋白水解物的抗氧化活性。结果表明：蛋白酶K、碱性酶和胰酶的水解度显著高于木瓜酶和风味酶。Alcalase对羊奶酪蛋白水解物的Fe2+螯合活性和超氧阴离子清除能力最强，Alcalase和木瓜酶对DPPH的清除活性最高，而Alcalase和木瓜酶对DPPH的清除能力差异不显著(p<；0.05)，说明Alcalase是最适合羊奶酪蛋白水解物产生抗氧化肽的水解酶。
Abstract Oxidation was related to the pathogenesis of human diseases. Adequate intake of antioxidant activity of food can reduce the levels of free radicals, prevent lipid peroxidation, and help the body against diseases. In the paper, casein from goat milk was hydrolyzed by five commercial proteases, namely, Alcalase, flavourzyme, papain, proteinase K and trypsin. The antioxidant activities of casein hydrolysates were assessed by evaluating hydrolysis degree, DPPH radical-scavenging activity, metal-chelating activity and superoxide radical scavenging activity. The results showed as follows: the DH of proteinase K, Alcalase, and trypsin were higher significantly than those of papain and flavourzyme. The Fe2+-chelation activity and superoxide radical scavenging activity of casein hydrolysates from goat milk by Alcalase was higher than the others, the DPPH scavenging activities of casein hydrolysates by Alcalase and papain was higher than the others and the DPPH scavenging activities by Alcalase and papain had no significant diffierence (p<0.05), so the optimal proteinase for hydrolysis casein from goat milk to produce antioxidant peptide was Alcalase.