Effect of Subinhibitory Concentrations of Plant-Derived Molecules in Increasing the Sensitivity of Multidrug-Resistant Salmonella enterica Serovar Typhimurium DT104 to Antibiotics
Effect of Subinhibitory Concentrations of Plant-Derived Molecules in Increasing the Sensitivity of Multidrug-Resistant Salmonella enterica Serovar Typhimurium DT104 to Antibiotics
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DOI：
10.1089/fpd.2009.0527
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发表时间：
2010-10-01
影响因子：
2.8
通讯作者：
Venkitanarayanan, Kumar
中科院分区：
文献类型：
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作者：
Johny, Anup Kollanoor;Hoagland, Thomas;Venkitanarayanan, Kumar
本研究调查了植物源性抗菌剂，即反式肉桂醛，β-间苯二酚，香芹酚，百里酚，丁香酚或其组合，在增加鼠伤寒沙门氏菌DT 104的敏感性的有效性5种抗生素。将亚抑制浓度的每种抗菌剂或其组合（含有低于单个亚抑制浓度的浓度）加入到补充有抗生素的胰蛋白酶大豆肉汤中，达到其各自的耐药性折点。将鼠伤寒沙门氏菌DT 104以类似于6 log CFU/mL的浓度接种到胰蛋白酶大豆肉汤中，并在37 ℃下孵育24小时前后测定生长（600 nm处的光密度）。包括适当的对照。测定一式两份样品，并将实验重复三次。反式肉桂醛增加了鼠伤寒沙门氏菌DT 104（p < 0.05）对所有五种抗生素，即氨苄青霉素，氯霉素，链霉素，磺胺甲恶唑和四环素的敏感性，从而使病原体对药物敏感。麝香草酚使病原体对除氨苄青霉素外的所有四种抗生素敏感，而香芹酚增加了对两种抗生素（菌株H3380的氯霉素和磺胺甲恶唑，菌株43的链霉素和磺胺甲恶唑）的敏感性。五种分子的组合比单个分子更有效（p < 0.05）使病原体对抗生素敏感。结果表明，这些天然分子单独和协同增加了鼠伤寒沙门氏菌DT 104对所有五种抗生素的敏感性，并证明了未来的研究，以控制使用这些植物分子的食品动物中的病原体的抗生素耐药性。
This study investigated the efficacy of plant-derived antimicrobials, namely, trans-cinnamaldehyde, beta-resorcylic acid, carvacrol, thymol, and eugenol or their combination, in increasing the sensitivity of Salmonella Typhimurium DT104 to five antibiotics. The subinhibitory concentrations of each antimicrobial or their combination containing concentrations lower than the individual subinhibitory concentrations were added to tryptic soy broth supplemented with antibiotics at their respective break points for resistance. Salmonella Typhimurium DT104 was inoculated into tryptic soy broth at similar to 6 log CFU/mL, and growth (optical density at 600 nm) was determined before and after incubation at 37 degrees C for 24 hours. Appropriate controls were included. Duplicate samples were assayed and the experiment was replicated three times. Trans-cinnamaldehyde increased the sensitivity of Salmonella Typhimurium DT104 (p < 0.05) toward all five antibiotics, namely, ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline, thereby making the pathogen susceptible to drugs. Thymol made the pathogen susceptible to all four antibiotics except ampicillin, whereas carvacrol increased the sensitivity to two antibiotics (chloramphenicol and sulfamethoxazole for strain H3380, and streptomycin and sulfamethoxazole for strain 43). The combination of five molecules was more effective than individual ones (p < 0.05) in rendering the pathogen susceptible to the antibiotics. Results indicate that these natural molecules individually and synergistically increased the sensitivity of Salmonella Typhimurium DT104 to all the five antibiotics, and justify future studies to control antibiotic resistance of the pathogen in food animals using these plant molecules.