Globular domain of adiponectin: promising target molecule for detection of atherosclerotic lesions
Globular domain of adiponectin: promising target molecule for detection of atherosclerotic lesions
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DOI：
10.2147/btt.s22863
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发表时间：
2011-01-01
影响因子：
4
通讯作者：
Mangge, Harald
中科院分区：
文献类型：
--
作者：
Almer, Gunter;Saba-Lepek, Matthias;Mangge, Harald
背景资料：脂联素是一种脂肪细胞特异性血浆蛋白，已被证明在动脉粥样硬化病变的发展过程中在受损的内皮细胞中积聚。在这项研究中，我们调查了不同的脂联素亚组分的潜力，特别强调球状脂联素（gAd）识别和可视化动脉粥样硬化lesions.Methods：重组小鼠gAd和全长脂联素亚组分（即，三聚体，六聚体和寡聚体形式）进行荧光标记。解剖喂食高胆固醇饮食的野生型和载脂蛋白E缺陷小鼠的主动脉，并与标记的生物标志物一起孵育。成像进行共聚焦激光扫描microscopic.Results：共聚焦激光扫描显微镜图像显示，gAd结合更强烈的动脉粥样硬化斑块比全长脂联素亚组分。此外，我们发现，gAd积累优先在内皮细胞和纤维帽面积的斑块。在这里，我们证明了第一次，gAd承认载脂蛋白E-缺陷mice.Conclusion主动脉节动脉粥样硬化斑块：这些结果表明，gAd，除了它的生理特性，也适合作为一个目标分子的前瞻性诊断策略，在成像动脉粥样硬化病变。
Background: Adiponectin, an adipocyte-specific plasma protein, has been shown to accumulate in injured endothelial cells during development of atherosclerotic lesions. In this study, we investigated the potential of different adiponectin subfractions with special emphasis on globular adiponectin (gAd) to recognize and visualize atherosclerotic lesions.Methods: Recombinant mouse gAd and subfractions of full-length adiponectin (ie, trimeric, hexameric, and oligomeric forms) were fluorescence-labeled. Aortas of wild-type and apoprotein E-deficient mice fed a high cholesterol diet were dissected and incubated with the labeled biomarkers. Imaging was performed using confocal laser scanning microscopy.Results: Confocal laser scanning microscopic images showed that gAd binds more strongly to atherosclerotic plaques than full-length adiponectin subfractions. Further, we showed that gAd accumulates preferentially in endothelial cells and the fibrous cap area of plaques. Here we demonstrate for the first time that gAd recognizes atherosclerotic plaques on aortic sections of apoprotein E-deficient mice.Conclusion: These results suggest that gAd, in addition to its physiological properties, is also suitable as a target molecule for prospective diagnostic strategies in imaging atherosclerotic lesions.