Pancreatic kallikrein gene expression: effects of glucocorticoids in vivo and in vitro.
Pancreatic kallikrein gene expression: effects of glucocorticoids in vivo and in vitro.
复制标题
胰腺激肽释放酶基因表达：糖皮质激素体内和体外的作用。
DOI：
10.1016/0016-5085(89)91510-2
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发表时间：
1989
期刊：
影响因子：
29.4
通讯作者：
Logsdon,CD
中科院分区：
文献类型：
--
作者：
Rosewicz,S;Logsdon,CD
我们在体内和体外研究了糖皮质激素在调节胰腺腺激肽释放酶基因表达中的作用。成年雄性大鼠肾上腺切除（Adx）。给予皮质酮颗粒以维持生理（Adx 1+）或高生理（Adx 3+）血浆皮质酮水平。胰激肽释放酶mRNA水平通过北方杂交检测，并通过狭缝印迹杂交定量。与假手术对照组相比，肾上腺切除术导致激肽释放酶mRNA增加75% ± 14%（n = 4）。这种增加被完全逆转的外源性皮质酮替代正常生理浓度。用高水平的皮质酮（Adx 3+）替代导致激肽释放酶mRNA水平下降至对照的53% ± 4%（n = 4）。在个体激肽释放酶mRNA水平和血浆皮质酮之间观察到显著负相关（r =-0.81，n = 12）。在体外，使用大鼠胰腺腺泡细胞系AR 42 J，地塞米松以时间和剂量依赖性方式降低激肽释放酶mRNA稳态水平。因此，这些数据表明，生理浓度的血浆皮质酮降低体内胰腺激肽释放酶mRNA水平，这是对胰腺腺泡细胞的直接影响。
We examined the role of glucocorticoids in the regulation of pancreatic glandular kallikrein gene expression in vivo and in vitro. Adult male rats were adrenalectomized (Adx). Corticosterone pellets were administered to maintain either physiologic (Adx 1+) or high physiologic (Adx 3+) plasma corticosterone levels. Pancreatic kallikrein mRNA levels were examined by Northern hybridization and quantitated by slot-blot hybridization. Adrenalectomy resulted in a 75% ± 14% (n = 4) increase in kallikrein mRNA as compared with sham-operated controls. This increase was completely reversed by exogenous corticosterone replacement to normal physiologic concentrations. Replacement with high corticosterone levels (Adx 3+) resulted in a decrease of kallikrein mRNA levels to 53% ± 4% (n = 4) of controls. A significant negative correlation was observed between individual kallikrein mRNA levels and plasma corticosterone (r = − 0.81, n = 12). In vitro, using the rat pancreatic acinar cell line AR42J, dexamethasone decreased kallikrein mRNA steady-state levels in a time- and dose-dependent manner. These data, therefore, indicate that physiologic concentrations of plasma corticosterone decrease pancreatic kallikrein mRNA levels in vivo, and that this is a direct effect on pancreatic acinar cells.