Giant Gold Nanowire Vesicle-Based Colorimetric and SERS Dual-Mode Immunosensor for Ultrasensitive Detection of Vibrio parahemolyticus
Giant Gold Nanowire Vesicle-Based Colorimetric and SERS Dual-Mode Immunosensor for Ultrasensitive Detection of Vibrio parahemolyticus
复制标题
基于巨型金纳米线囊泡的比色和 SERS 双模式免疫传感器，用于超灵敏检测副溶血弧菌
DOI：
10.1021/acs.analchem.8b00292
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发表时间：
2018-05-15
影响因子：
7.4
通讯作者：
Chen, Tao
中科院分区：
文献类型：
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作者：
Guo, Zhiyong;Jia, Yaru;Chen, Tao
传统的副溶血性弧菌（Vibrio parahemolyticus，VP）检测方法操作繁琐，灵敏度低，限制了VP的实际应用。本研究以锚定金纳米线（AuNW）的巨Au囊泡为探针，建立了一种简单高效的比色法和表面增强拉曼散射（Sers）双模式免疫传感器，用于VP的灵敏检测。由于巨Au囊泡具有较大的比表面积和特殊的中空结构，银囊泡的银染色容易使其颜色发生鲜明的变化，从而进一步放大Sers信号。进一步使用t检验来确定来自比色法和Sers的两组数据是否彼此显著不同。结果表明，两种方法所得数据无显著性差异。两组数据可以相互验证，避免误报和漏报。所设计的色度Sers双模传感器在食品安全检测、个人医疗保健、现场环境监测等领域具有广阔的应用前景。
Conventional methods for the detection of Vibrio parahemolyticus (VP) usually need tedious, labor-intensive processes, and have low sensitivity, which further limits their practical applications. Herein, we developed a simple and efficient colorimetry and surface-enhanced Raman scattering (SERS) dual-mode immunosensor for sensitive detection of VP, by employing giant Au vesicles with anchored tiny gold nanowires (AuNW) as a smart probe. Due to the larger specific surface and special hollow structure of giant Au vesicles, silver staining would easily lead to vivid color change for colorimetric analysis and further amplify SERS signals. The t-test was further used to determine if two sets of data from colorimetry and SERS were significantly different from each other. The result shows that there was no significant difference between data from the two methods. Two sets of data can mutually validate each other and avoid false positive and negative detection. The designed colorimetry SERS dual-mode sensor would be very promising in various applications such as food safety inspection, personal healthcare, and on-site environmental monitoring.