Fusion of nasal swellings in the mouse embryo: surface coat and initial contact.
Fusion of nasal swellings in the mouse embryo: surface coat and initial contact.
复制标题
小鼠胚胎鼻肿胀的融合：表面涂层和初始接触。
作者：
J. Gaare;J. Langman
用电子显微镜对12日龄小鼠胚胎的鼻区进行了检查，以确定内侧和外侧鼻部肿胀之间的上皮融合是否涉及表面层和膜的特化。用钌红分析了涂层的分布情况。在接触之前，推测融合区的鼻部肿胀的上皮衬里上总是有一层表层，而且融合区的表层通常比非融合区的厚。在最初的接触点，在接触的表面细胞之间，涂层是一层薄膜，这表明它可能参与了上皮细胞的接触。内侧和外侧鼻部肿胀的细胞之间的初始接触是通过一个表面细胞到相对的表面细胞相交的表面的短突起来实现的。接触膜之间的距离约为6-10 nm，电子密度增加，可能在鼻部肿胀之间提供了牢固的粘连。
The nasal region of 12-day-old mouse embryos was examined with the electron microscope to determine whether a surface coat and membrane specializations are involved in epithelial fusion between the medial and lateral nasal swellings. Ruthenium red was used to examine the distribution of the surface coat. Prior to contact, a surface coat is always present over the epithelial linings of the nasal swellings in the region of presumptive fusion, and it is often heavier in the fusing than in the non-fusing regions. At the point of initial contact, the coat is present as a thin film between touching superficial cells, suggesting that it may mediate epithelial contact. The initial contact between the cells of the medial and lateral nasal swellings is made by short projections from one superficial cell to the surface of an opposing superficial cells meet. The contacting membranes, which are separated by a distance of approximately 6-10 nm and show an increased electron-density, probably provide a firm adhesion between the nasal swellings.