A novel cDNA encoding a human homologue of ribosomal protein L29
A novel cDNA encoding a human homologue of ribosomal protein L29
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DOI：
10.1016/0167-4781(95)00224-3
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发表时间：
1996-03-01
期刊：
影响因子：
--
通讯作者：
Waye, MMY
中科院分区：
文献类型：
--
作者：
Law, PTW;Tsui, SKW;Waye, MMY
在对人类心脏cDNA克隆进行大规模部分测序时，发现了一个在DNA和氨基酸序列上与大鼠核糖体蛋白L29非常相似的新克隆。该 cDNA 编码的蛋白质推导分子量为 17 751（159 个氨基酸）。它与来自大鼠核糖体大亚基的蛋白质 L29 有 80.4% 同源性，并且与酵母 YL43 相关。该推定蛋白被命名为人核糖体蛋白 L29 (hRPL29)。 hRPL29 的碱性残基远多于酸性残基。大量的带电残基使蛋白质非常亲水，推导的 pi 为 12.16。内部重复序列已在许多核糖体蛋白中得到表征，并且发现 KAKAKAKA 串联重复序列是 hRPL29 所独有的。通过Southern印迹分析基因组织表明，在hrpL29的大约10个拷贝中，除了一个之外，所有拷贝都是假基因。 Northern 分析表明，编码人类 L29 的 mRNA 约为。长度为 800 个碱基对。 hrpL29 的内含子也已使用人类基因组 DNA 作为模板，通过聚合酶链式反应进行了克隆和测序。
During the large scale partial sequencing of human heart cDNA clones, a novel clone which is very similar to the rat ribosomal protein L29 in both DNA and amino acid sequences was found. The cDNA encodes a protein with a deduced molecular weight of 17 751 (159 aa). It shows 80.4% homology to protein L29 from the large ribosomal subunit of rat and is related to yeast YL43. The putative protein was named human ribosomal protein L29 (hRPL29). hRPL29 has a large excess of basic residues over acidic ones. The large amount of charged residues makes the protein very hydrophilic and the protein has a deduced pi of 12.16. Internal repeats have been characterised in many ribosomal proteins and a tandem repeat of KAKAKAKA was found to be unique to hRPL29. Analysis of gene organisation by Southern blotting shows that of the approximate 10 copies of hrpL29, all but one are pseudogenes. Northern analysis indicated that the mRNA that encodes human L29 is approx. 800 base pairs in length. An intron of hrpL29 has also been cloned and sequenced by polymerase chain reaction using human genomic DNA as the template.