A noncanonical endocytic pathway is involved in the internalization of 3 μm polystyrene beads into HeLa cells
A noncanonical endocytic pathway is involved in the internalization of 3 μm polystyrene beads into HeLa cells
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3 μm 聚苯乙烯珠内化到 HeLa 细胞中涉及非经典内吞途径
DOI：
10.1039/d2bm01353c
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发表时间：
2022
影响因子：
6.6
通讯作者：
Futaki Shiroh
中科院分区：
文献类型：
--
作者：
Hirose Hisaaki;Maekawa Masashi;Ida Hiroki;Kuriyama Masashi;Takahashi Yasufumi;Futaki Shiroh
不同大小和来源的细胞外微粒可以被细胞摄取，影响其功能。了解细胞摄取过程对于了解这些颗粒的细胞效应以及开发控制其内化的方法至关重要。虽然巨噬细胞吞噬是细胞摄取大于0.2μm的颗粒的可能途径，但它对非吞噬细胞的细胞摄取的贡献是有争议的。以3μm聚苯乙烯微珠为模型粒子，研究其在非吞噬细胞中的摄取模式。使用共聚焦、扫描电子和扫描离子电导显微镜分析以及抑制剂研究来评估细胞摄取。我们的结果表明，3μm小球被HeLa细胞通过肌动蛋白、胆固醇和膜突起依赖的非典型内吞途径摄取，不同于典型的大吞饮和吞噬途径。我们的工作为研究细胞对细胞外细颗粒的摄取提供了一个框架。
Extracellular fine particles of various sizes and origins can be taken up by cells, affecting their function. Understanding the cellular uptake processes is crucial for understanding the cellular effects of these particles and the development of means to control their internalization. Although macropinocytosis is a possible pathway for the cellular uptake of particles larger than 0.2 μm, its contribution to cellular uptake in non-phagocytic cells is controversial. Using 3 μm polystyrene beads as a model particle, we aimed to assess the detailed modes of their cellular uptake by non-phagocytic HeLa cells. Cellular uptake was assessed using confocal, scanning electron, and scanning ion conductance microscopy analyses, together with inhibitor studies. Our results revealed that 3 μm beads were taken up by HeLa cells by an actin-, cholesterol-, and membrane protrusions-dependent noncanonical endocytic pathway, different from the canonical macropinocytic and phagocytic pathways. Our work provides a framework for studying the cellular uptake of extracellular fine particles.