Widespread distribution of deletions of the bgl operon in natural isolates of Escherichia coli.
Widespread distribution of deletions of the bgl operon in natural isolates of Escherichia coli.
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bgl 操纵子缺失在大肠杆菌的天然分离株中广泛分布。
DOI：
10.1093/oxfordjournals.molbev.a040499
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发表时间：
1988
影响因子：
10.7
通讯作者：
Hall,BG
中科院分区：
文献类型：
--
作者：
Hall,BG
大肠杆菌的bgl（β-葡萄糖苷利用）操纵子的缺失最初在几种很少发生的利用纤维二糖的天然分离物中检测到。在这里，我表明，bgl缺失存在于95%的从不同来源获得的Cel+分离株。它们也存在于两个不同的E.杆菌在大肠杆菌中至少存在三种bgl缺失形式。大肠杆菌种群。在最常见的版本中，E. coliK 12中特异的6.5kb DNA片段。在另一个版本中，相似长度的缺失不被相同序列替换。第三个版本涉及缺失约14 kb而不存在替换片段。这些缺失的分布表明，版本1缺失发生在大肠杆菌历史的早期。在大肠杆菌的Cel+菌株中也可能存在bgl缺失的选择。杆菌在E.为大肠杆菌天然群体内的重组提供了证据。
A deletion that includes the bgl (beta-glucoside utilization) operon of Escherichia coli was originally detected in several rarely occurring natural isolates that utilize cellobiose. Here I show that bgl deletions are present in 95% of the Cel+ isolates obtained from diverse sources. They are also present in 29% of the Cel- strains in two different collections of natural isolates of E. coli. At least three versions of bgl deletions are present in E. coli populations. In the most common version approximately 8 kb of DNA around the bgl region of E. coli K12 is replaced by a specific 6.5-kb DNA fragment. In another version a deletion of similar length is not replaced by the same sequence. A third version involves deletion of approximately 14 kb without the replacement fragment being present. The distribution of these deletions suggests that the version 1 deletion occurred very early in the history of E coli. It also appears likely that there is selection for bgl deletions in Cel+ strains of E. coli. The presence of the version 1 deletion within distantly related phylogenetic groups of E. coli provides evidence for recombination within natural populations of E coli.