Regression of atherosclerosis induced by liver-directed gene transfer of apolipoprotein A-I in mice
Regression of atherosclerosis induced by liver-directed gene transfer of apolipoprotein A-I in mice
复制标题
DOI：
10.1161/01.cir.100.17.1816
复制
发表时间：
1999-10-26
期刊：
影响因子：
37.8
通讯作者：
Rader, DJ
中科院分区：
文献类型：
--
作者：
Tangirala, RK;Tsukamoto, K;Rader, DJ
背景-载脂蛋白（apo）A-1诱导先前存在的动脉粥样硬化病变消退的能力尚未确定，并且动脉粥样硬化消退的小鼠模型尚未报道。方法和结果-LDL受体缺陷小鼠喂食西式饮食5周以诱导动脉粥样硬化病变。静脉注射编码人apoA-I的第二代重组腺病毒或对照腺病毒，以在肝脏中表达apoA-I。注射后3天，注射表达apoA-I的腺病毒的小鼠的总apoA-I水平为216+/-16.0 mg/dL，而对照病毒注射小鼠的总apoA-I水平为68.0+/-3.0 mg/dL（P
Background-The ability of apolipoprotein (apo)A-l to induce regression of preexisting atherosclerotic lesions has not been determined, and a mouse model of atherosclerosis regression has not yet been reported.Methods and Results-LDL receptor-deficient mice were fed a western-type diet for 5 weeks to induce atherosclerotic lesions. A second-generation recombinant adenovirus encoding human apoA-I or a control adenovirus were injected intravenously in order to express apoA-I in the liver. Three days after injection, total apoA-I levels in mice injected with the apoA-I-expressing adenovirus were 216+/-16.0 mg/dL, compared with 68.0+/-3.0 mg/dL in control virus-injected mice (P