Myosin II motor activity in the lateral amygdala is required for fear memory consolidation
Myosin II motor activity in the lateral amygdala is required for fear memory consolidation
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DOI：
10.1101/lm.024042.111
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发表时间：
2012-01-01
影响因子：
2
通讯作者：
Rumbaugh, Gavin
中科院分区：
文献类型：
--
作者：
Gavin, Cristin F.;Rubio, Maria D.;Rumbaugh, Gavin
学习诱导肌动蛋白细胞骨架的动态变化，这是支持记忆形成所必需的。然而，在学习和记忆过程中，调节丝状肌动蛋白（F-actin）动力学的分子机制尚不清楚。Myosin II马达在包括树突棘在内的富含肌动蛋白的生长结构中高度表达，我们最近发现这些分子机器动员f -肌动蛋白响应突触刺激和海马的学习。在这项研究中，我们报道了大鼠外侧杏仁核（LA）中的肌球蛋白II运动对恐惧记忆的形成至关重要。训练前输注肌球蛋白II抑制剂blebbistatin （blebb）破坏了长期记忆，而短期记忆未受影响。有趣的是，训练后和测试前注射对记忆形成没有影响，这表明Myosin II运动在学习期间或学习后不久就会促进记忆巩固。这些数据支持Myosin II运动力产生是支持哺乳动物中枢神经系统记忆巩固的一般机制的观点。
Learning induces dynamic changes to the actin cytoskeleton that are required to support memory formation. However, the molecular mechanisms that mediate filamentous actin (F-actin) dynamics during learning and memory are poorly understood. Myosin II motors are highly expressed in actin-rich growth structures including dendritic spines, and we have recently shown that these molecular machines mobilize F-actin in response to synaptic stimulation and learning in the hippocampus. In this study, we report that Myosin II motors in the rat lateral amygdala (LA) are essential for fear memory formation. Pretraining infusions of the Myosin II inhibitor, blebbistatin (blebb), disrupted long term memory, while short term memory was unaffected. Interestingly, both post-training and pretesting infusions had no effect on memory formation, indicating that Myosin II motors operate during or shortly after learning to promote memory consolidation. These data support the idea that Myosin II motor-force generation is a general mechanism that supports memory consolidation in the mammalian CNS.