RNA interference of insulin-related peptide and neuroparsins affects vitellogenesis in the desert locust Schistocerca gregaria
RNA interference of insulin-related peptide and neuroparsins affects vitellogenesis in the desert locust Schistocerca gregaria
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DOI：
10.1016/j.peptides.2010.11.008
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发表时间：
2011-03-01
期刊：
影响因子：
3
通讯作者：
Vanden Broeck, Jozef
中科院分区：
文献类型：
--
作者：
Badisco, Liesbeth;Marchal, Elisabeth;Vanden Broeck, Jozef
“经典”的昆虫激素，保幼激素和20-羟基蜕皮激素，可以刺激几种昆虫的成年雌性的卵黄发生和/或卵巢发育。越来越多的证据也表明，肽类激素，如胰岛素相关肽（IRP）和神经肽（NP）在女性生殖生理学中起着至关重要的作用。特别是在双翅目物种中，IRP信号已被证明可以调节雌性生殖事件。第一个NP最初从飞蝗（Locusta migratoria）中鉴定为延迟卵黄发生的抗促性腺因子。而且。NP家族成员显示与脊椎动物胰岛素样生长因子结合蛋白（IGFBPs）的N-末端结构域的序列相似性。本研究利用RNA干扰技术研究了IRP和NPs对雌性沙漠蝗生殖系统的调控作用。编码一个IRP（Scg-IRP）和四个NP（Scg-NPs）的cDNA先前已从S.聚集。在成年雌性沙漠蝗虫中诱导了RNAi介导的Scg-NP或Scg-IRP转录水平的敲低，并分析了随后的影响。Scg-NPs或Scg-IRP的敲低分别以正和负的方式影响卵黄蛋白原的转录水平和卵母细胞的生长。目前的发现表明Scg-NPs和Scg-IRP在控制卵黄蛋白原合成中的作用。一个合理的假设是，Scg-IRP可能作为一个传感器的营养和代谢状态，决定是否可以发生卵黄。在两个RNAi实验中，相同的过程以相反的方式受到影响，这为Scg-NPs和Scg-IRP的拮抗作用提供了额外的论据。(C)2010年爱思唯尔公司All rights reserved.
The 'classic' insect hormones, juvenile hormone and 20-hydroxyecdysone, can stimulate vitellogenesis and/or ovarian development in adult females of several insect species. Accumulating evidence also indicates a crucial role in female reproductive physiology for peptide hormones, such as insulin-related peptides (IRPs) and neuroparsins (NPs). Especially in dipteran species, IRP signaling has been shown to regulate female reproductive events. The first NP was originally identified from the migratory locust (Locusta migratoria) as an antigonadotropic factor that delayed vitellogenesis. Moreover. NP family members display sequence similarities with the N-terminal domain of vertebrate insulin-like growth factor binding proteins (IGFBPs). In the current study, RNA interference (RNAi) was employed to investigate the possible involvement of IRP and NPs in the control of the female desert locust (Schistocerca gregaria) reproductive system. The cDNAs encoding an IRP (Scg-IRP) and four NPs (Scg-NPs) had previously been cloned from S. gregaria. An RNAi-mediated knock-down of either Scg-NP or Scg-IRP transcript levels was induced in adult female desert locusts and the subsequent effects were analyzed. Knock-down of the Scg-NPs or Scg-IRP affected vitellogenin transcript levels and oocyte growth in a positive and negative way, respectively. The current findings are indicative for a role of Scg-NPs and Scg-IRP in the control of vitellogenin synthesis. A plausible hypothesis is that Scg-IRP may act as a sensor of the nutritional and metabolic status that determines whether vitellogenesis can occur. That the same processes were affected in opposite ways in both RNAi experiments offers an extra argument for antagonizing roles of Scg-NPs and Scg-IRP. (C) 2010 Elsevier Inc. All rights reserved.