Current approaches for detection of hydrogen sulfide and persulfidation in biological systems.
Current approaches for detection of hydrogen sulfide and persulfidation in biological systems.
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DOI：
10.1016/j.plaphy.2020.08.006
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发表时间：
2020-08
期刊：
影响因子：
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通讯作者：
Didi Zhao;Jing Zhang;Mingjian Zhou;Heng Zhou;C. Gotor;L. Romero;Jie Shen;Xingxing Yuan;Yanjie Xie
中科院分区：
文献类型：
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作者：
Didi Zhao;Jing Zhang;Mingjian Zhou;Heng Zhou;C. Gotor;L. Romero;Jie Shen;Xingxing Yuan;Yanjie Xie
在过去的几十年里，硫化氢（H2S）作为气体信号分子参与了生物系统中多种细胞和生理过程。近年来，相当多的研究强调了这种氧化还原调节分子的信号作用是通过过硫酸化发生的，过硫酸化是蛋白质半胱氨酸残基通过巯基形成过硫共价加成的翻译后修饰。然而，我们目前对生物系统中H2S的检测和过硫化的认识仍然滞后。本文综述了目前测量生物系统中H2S和过硫水平的方法。同时，提出并讨论了植物研究中可能存在的潜在干扰。
The past decades have witnessed hydrogen sulfide (H2S) serving as gaseous signaling molecule participating in diverse cellular and physiological processes in biological systems. Recently, a considerable number of studies highlight the signaling role of this redox-regulating molecule occurs via persulfidation, which is a post-translation modification of protein cysteine residues by covalent addition of thiol group form persulfide. However, our current understanding on detection of H2S and persulfidation in biological systems still lags behind. This review aims to summarize current approaches for measuring H2S and persulfidated levels in biological systems. Meanwhile, potential interference may exist in plant research has been proposed and discussed.