Stabilizing breathing pattern using local mechanical vibrations: comparison of deterministic and stochastic stimulations in rodent models of apnea of prematurity.
Stabilizing breathing pattern using local mechanical vibrations: comparison of deterministic and stochastic stimulations in rodent models of apnea of prematurity.
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DOI：
10.1007/s13534-021-00203-x
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发表时间：
2021-11
影响因子：
4.6
通讯作者：
Suki B
中科院分区：
文献类型：
--
作者：
Zeldich D;Bou Jawde S;Herrmann J;Arnaout L;Griffin M;Grunfeld N;Zhang Y;Krishnan R;Bartolák-Suki E;Suki B
机械刺激已被证明可以减少早产儿呼吸暂停（AOP），这是早产儿的一个主要问题。以前的工作表明，潜在的机制是随机共振，放大的阈下信号的随机刺激。我们假设，呼吸暂停长度减少背后的机制可能不仅仅是一种随机现象，并建议纯粹的确定性，非随机的机械刺激可能同样有效。对小鼠和大鼠进行麻醉、气管造口术和机械通气以停止自主呼吸。两个由微控制器控制的微型马达连接在腹部周围。暂停通气，施加刺激，并测量啮齿动物第一次自主呼吸的时间（T）。在小鼠中将六种光谱不同的信号彼此进行比较并与无刺激对照进行比较。然后将减少呼吸暂停的最成功的确定性刺激（D）与具有可比幅度和频率的伪随机噪声（PRN）信号进行比较。还测量了CO2%、CO2稳定时间（Ts）、氧饱和度（SpO2%）和T。对于中等和高振幅，与无刺激相比，D显著降低了T。PRN也降低了T，而D和PRN之间没有差异。此外，两种刺激均显著降低Ts，各刺激之间无显著差异。然而，D或PRN对SpO2%没有影响。D和PRN之间缺乏差异导致了在年轻大鼠中将相同的D与带限白色噪声（WN）信号进行比较的额外系列实验。D和WN均显示出显着降低T，其中D在减少呼吸暂停方面显示出统计学优势。我们进一步推测，无论是确定性和随机性的机械刺激诱导某种形式的mechanotransduction这是他们的疗效负责，我们的研究结果表明，机械刺激可能是有效的治疗AOP。在线版本包含补充材料，可通过10.1007/s13534-021-00203-x获得。
Mechanical stimulation has been shown to reduce apnea of prematurity (AOP), a major concern in preterm infants. Previous work suggested that the underlying mechanism is stochastic resonance, amplification of a subthreshold signal by stochastic stimulation. We hypothesized that the mechanism behind the reduction of apnea length may not be a solely stochastic phenomenon, and suggest that a purely deterministic, non-random mechanical stimulation could be equally as effective. Mice and rats were anesthetized, tracheostomized, and mechanically ventilated to halt spontaneous breathing. Two miniature motors controlled by a microcontroller were attached around the abdomen. Ventilation was paused, stimulations were applied, and the time to the rodent’s first spontaneous breath (T) was measured. Six spectrally different signals were compared to one another and the no-stimulation control in mice. The most successful deterministic stimulation (D) at reducing apnea was then compared to a pseudo-random noise (PRN) signal of comparable amplitude and frequency. CO2%, CO2 stabilization time (Ts), O2 saturation (SpO2%), and T were also measured. D significantly reduced T compared to no stimulation for medium and high amplitudes. PRN also reduced T, without a difference between D and PRN. Furthermore, both stimulations significantly reduced Ts with no significant differences between the respective stimulations. However, there was no effect of D or PRN on SpO2%. The lack of differences between D and PRN led to an additional series of experiment comparing the same D to a band-limited white noise (WN) signal in young rats. Both D and WN were shown to significantly reduce T, with D showing statistical superiority in reduction of apnea. We further speculate that both deterministic and stochastic mechanical stimulations induce some form of mechanotransduction which is responsible for their efficacy, and our findings suggest that mechanical stimulation may be effective in treating AOP. The online version contains supplementary material available at 10.1007/s13534-021-00203-x.
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影响因子：
3.4
作者：
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通讯作者：
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