Involvement of the Wnt/β-catenin pathway in neurectoderm architecture in Platynereis dumerilii
Involvement of the Wnt/β-catenin pathway in neurectoderm architecture in Platynereis dumerilii
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DOI：
10.1038/ncomms2915
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发表时间：
2013-05-01
影响因子：
16.6
通讯作者：
Vervoort, Michel
中科院分区：
文献类型：
--
作者：
Demilly, Adrien;Steinmetz, Patrick;Vervoort, Michel
信号通路对于双侧动物中枢神经系统(CNS)的正确发育至关重要。在这里，我们发现在环节动物Platynereis umerilii的CNS中，神经前体细胞(NPC)位于腹中线附近，并表达轴蛋白，轴蛋白是Wnt/β-catenin途径的负调节因子。使用药物抑制剂，我们观察到Wnt/β-连环蛋白是增殖的神经前体细胞和分化的神经元之间过渡所必需的。我们还发现Rho相关激酶(Rho-Association Kinase，ROK)是神经直肠胚层形态发生和腹中线形成所必需的，并间接影响神经前体细胞的分布和轴突支架的发育。此外，七个属于平面细胞极性(PCP)途径的基因在发育中的扁平线虫神经外胚层中表达，表明它参与了其形态发生。与以前在脊椎动物中的研究相比，我们的数据表明，Wnt/β-catenin途径参与控制神经细胞的增殖/分化是双边动物的祖先。
Signalling pathways are essential for the correct development of the central nervous system (CNS) in bilaterian animals. Here we show that in the CNS of the annelid Platynereis dumerilii, neural progenitor cells (NPCs) are located close to the ventral midline and express axin, a negative regulator of the Wnt/beta-catenin pathway. Using pharmacological inhibitors, we observe that Wnt/beta-catenin is required for the transition between proliferating NPCs and differentiating neurons. We also show that the Rho-associated kinase (Rok) is necessary for neurectoderm morphogenesis and ventral midline formation, and indirectly affects the distribution of the NPCs and the development of axonal scaffolds. Moreover, seven genes belonging to the planar cell polarity (PCP) pathway are expressed in the developing Platynereis neurectoderm, suggesting an involvement in its morphogenesis. When compared with previous studies in vertebrates, our data suggest that the involvement of the Wnt/beta-catenin pathway in the control of neural cell proliferation/differentiation is ancestral to bilaterians.