Immune responses to Brugia malayi paramyosin in rodents after DNA vaccination
Immune responses to Brugia malayi paramyosin in rodents after DNA vaccination
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DOI：
10.1016/s0264-410x(99)00182-6
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发表时间：
1999-08-20
期刊：
影响因子：
5.5
通讯作者：
Weil, GJ
中科院分区：
文献类型：
--
作者：
Li, BW;Zhang, SR;Weil, GJ
用重组马来丝虫副肌球蛋白（BM 5）免疫沙鼠，诱导对该丝虫的部分免疫。本研究检测了肌肉注射编码BM 5的质粒DNA的免疫效果。DNA免疫的小鼠产生强的抗体和细胞介导的副肌球蛋白的反应。在沙鼠中测试了用BM 5进行DNA疫苗接种的保护活性。接种疫苗的沙鼠产生了强烈的抗体反应副肌球蛋白，但成年蠕虫的复苏挑战后，接种疫苗的动物相对于对照组没有减少。这些研究表明，DNA疫苗接种可以诱导啮齿动物对丝虫抗原的免疫应答。需要进一步努力，以实现利用这一有前途的新技术诱导对丝虫病的保护性免疫的目标。(C)1999爱思唯尔科技有限公司。保留所有权利。
Immunization with recombinant Brugia malayi paramyosin protein (BM5) induces partial immunity to this filarial nematode in jirds. The present study examined the effects of intramuscular immunization with plasmid DNA that encodes BM5. DNA-immunized mice produced strong antibody and cell-mediated responses to paramyosin. The protective activity of DNA vaccination with BM5 was tested in jirds. Vaccinated jirds produced strong antibody responses to paramyosin, but adult worm recoveries after challenge were not decreased in vaccinated animals relative to controls. These studies show that DNA vaccination can induce immune responses to filarial antigens in rodents. Further efforts will be needed to achieve the goal of inducing protective immunity to filariasis with this promising new technology. (C) 1999 Elsevier Science Ltd. All rights reserved.