Delayed protection of Ferulic acid in isolated hearts and cardiomyocytes: Upregulation of heat-shock protein 70 via NO-ERK1/2 pathway
Delayed protection of Ferulic acid in isolated hearts and cardiomyocytes: Upregulation of heat-shock protein 70 via NO-ERK1/2 pathway
复制标题
阿魏酸对离体心脏和心肌细胞的延迟保护：通过 NO-ERK1/2 途径上调热休克蛋白 70
DOI：
10.1016/j.jff.2017.04.012
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发表时间：
2017-07-01
影响因子：
5.6
通讯作者：
He, Ming
中科院分区：
文献类型：
--
作者：
Liao, Zhangping;He, Huan;He, Ming
本研究调查了阿魏酸（FA）的延迟保护作用，阿魏酸是一种常见于水果、蔬菜和全谷物中的天然多酚。即使在开始I/R之前用标准食物代替含FA食物27小时，喂食FA饮食的小鼠心脏也能免受缺血/再灌注（I/R）损伤。FA显示心脏功能恢复加快，梗死面积缩小。这些发现在缺氧/复氧前24小时用FA预处理的心肌细胞中得到证实。此外，FA显著上调HSP 70表达。选择性敲低HSP 70，FA处理不能产生I/R抗性。此外，FA诱导NO产生增加，并激活ERK 1/2。NOS抑制剂L-NAME或ERK 1/2抑制剂PD 184352可阻断FA的延迟保护作用。用L-NAME或PD 184352处理抑制HSP 70上调。总之，FA处理导致延迟的心肌保护作用，这是通过上调HSP 70通过NO-ERK 1/2途径介导的。(C)2017爱思唯尔有限公司版权所有
This study investigated delayed protection of Ferulic acid (FA), a natural polyphenol commonly found in fruits, vegetables and whole grains. Hearts of mice that were fed a FA diet were protected against ischemia/reperfusion (I/R) injury, even if FA-containing chow was replaced with standard chow for 27 h before initiating I/R. FA showed an increased recovery in cardiac function and reduction in infarct size. These findings were confirmed in cardiomyocytes that were pretreated with FA, 24 h before anoxia/reoxygenation. Moreover, FA significantly upregulated HSP70 expression. Selective knockdown of HSP70, FA treatment failed to render resistance to I/R. Additionally, FA induced an increase in NO production, and activated ERK1/2. The delayed protection of FA was abolished by NOS inhibitor L-NAME, or ERK1/2 inhibitor PD184352. Treatment with L-NAME or PD184352 inhibited HSP70 upregulation. In conclusion, FA treatment results in delayed cardioprotection against I/R, which is mediated through upregulation of HSP70 via NO-ERK1/2 pathway. (C) 2017 Elsevier Ltd. All rights reserved.