The effect of transforming growth factor-beta on mechanical properties of the fibrous tissue regenerated in the patellar tendon after resecting the central portion
The effect of transforming growth factor-beta on mechanical properties of the fibrous tissue regenerated in the patellar tendon after resecting the central portion
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DOI：
10.1016/j.clinbiomech.2005.05.012
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发表时间：
2005-11-01
影响因子：
1.8
通讯作者：
Hayashi, K
中科院分区：
文献类型：
--
作者：
Anaguchi, Y;Yasuda, K;Hayashi, K
背景没有研究者研究应用生长因子对切除中央部分后肌腱中的体内组织再生的影响。本研究的目的是阐明应用转化生长因子（TGF）-β 1是否增加髌腱中央部分切除后再生组织的力学性能。将30只雌性家兔分成3组，每组10只，分别在髌腱中央切除宽3 mm、长10 mm的腱质。在组I中，将溶解在0.1-ml盐水中的5-ng TGF-β 1注射到髌腱中的切除部分中。在第二组中，只有0.1毫升的生理盐水注入切除部分。在第三组中，没有注射任何药物。术后6周处死所有动物。对髌骨肌腱中的再生组织和未切除的肌腱组织进行了力学和组织学评估。结果。关于再生组织，第I组的切线模量和拉伸强度显著大于第II组和第III组，而第II组和第III组之间的这些参数没有显著差异。解释。TGF-β 1的应用显著增加了切除中心部分后髌腱中再生的纤维组织的切线模量和拉伸强度。本研究为TGF-β 1在体内肌腱再生中的应用提供了重要的基础信息。(c)2005爱思唯尔有限公司保留所有权利。
Background. No investigators have studied the effects of an application of growth factors on the in vivo tissue regeneration in the tendon after resecting the central portion. The purpose of this study is to clarify whether an application of transforming growth factor (TGF)-betal increases the mechanical properties of the regenerated tissue in the patellar tendon after resecting the central portion.Methods. Thirty female rabbits were divided into three groups, after a 3 mm wide and 10 mm long tendon substance was resected from the central portion in the patellar tendon. In Group I, 5-ng TGF-betal dissolved in 0.1-ml saline was injected into the resected portion in the patellar tendon. In Group II, only 0.1-ml saline was injected into the resected portion. In Group III, nothing was injected. All animals were sacrificed at 6 weeks after surgery. Mechanical and histological evaluations were made concerning the regenerated tissue and the unresected tendon tissue in the patellar tendon.Findings. Concerning the regenerated tissue, the tangent modulus and the tensile strength of Group I were significantly greater than those of Groups II and III, while there were no significant differences in these parameters between Groups II and III.Interpretation. The application of TGF-betal significantly increases the tangent modulus and the tensile strength of the fibrous tissue regenerated in the patellar tendon after resecting the central portion. This study has provided basic important information on the utility of TGF-betal in the in vivo tendon regeneration. (c) 2005 Elsevier Ltd. All rights reserved.