Analysis of the CYP51 gene and encoded protein in propiconazole-resistant isolates of Mycosphaerella fijiensis
Analysis of the CYP51 gene and encoded protein in propiconazole-resistant isolates of Mycosphaerella fijiensis
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DOI：
10.1002/ps.1770
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发表时间：
2009-08-01
影响因子：
4.1
通讯作者：
Arango, Rafael
中科院分区：
文献类型：
--
作者：
Canas-Gutierrez, Gloria P.;Angarita-Velasquez, Monica J.;Arango, Rafael
背景：斐济球腔菌引起香蕉和大蕉最重要的病害-黑叶斑病。疾病控制主要是通过应用内吸性杀真菌剂，包括甾醇脱甲基化抑制剂（DMI）。它们的大量使用有利于耐药菌株的出现。结果：本研究在11 M的大肠杆菌中分离到CYP 51基因，并进行了序列测定。fijiensis菌株对丙环唑（最广泛使用的杀菌剂之一）表现出不同程度的体外敏感性。发现了6个可能与该杀菌剂敏感性丧失相关的突变：Y136 F，A313 G，Y 461 D，Y 463 D，Y 463 H和Y 463 N。使用从结核分枝杆菌（Zoff.）的晶体结构构建的蛋白质的同源模型分析突变。莱曼和诺伊曼。此外，在13 M中测定基因表达。结论：该菌株甾醇14 α-脱甲基酶基因序列发生了一些变化，这些变化与其它真菌对唑类杀菌剂的抗性有关。未发现基因表达与丙环唑耐药相关。(C)2009年化学工业协会
BACKGROUND: Mycosphaerella fijiensis Morelet causes black sigatoka, the most important disease in bananas and plantains. Disease control is mainly through the application of systemic fungicides, including sterol demethylation inhibitors (DMIs). Their intensive use has favoured the appearance of resistant strains. However, no studies have been published on the possible resistance mechanisms.RESULTS: In this work, the CYP51 gene was isolated and sequenced in 11 M. fijiensis strains that had shown different degrees of in vitro sensitivity to propiconazole, one of the most widely used DMI fungicides. Six mutations that could be related to the loss in sensitivity to this fungicide were found: Y136F, A313G, Y461D, Y463D, Y463H and Y463N. The mutations were analysed using a homology model of the protein that was constructed from the crystallographic structure of Mycobacterium tuberculosis (Zoff.) Lehmann & Neumann. Additionally, gene expression was determined in 13 M. fijiensis strains through quantitative analysis of products obtained by RT-PCR.CONCLUSION: Several changes in the sequence of the gene encoding sterol 14 alpha-demethylase were found that have been described in other fungi as being correlated with resistance to azole fungicides. No correlation was found between gene expression and propiconazole resistance. (C) 2009 Society of Chemical Industry