Molecular cytogenetic characterization of a new wheat-rye 4R chromosome translocation line resistant to powdery mildew
Molecular cytogenetic characterization of a new wheat-rye 4R chromosome translocation line resistant to powdery mildew
复制标题
抗白粉病小麦-黑麦4R染色体易位新系的分子细胞遗传学特征
DOI：
10.1007/s10577-013-9366-8
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发表时间：
2013-07-01
影响因子：
2.6
通讯作者：
Xu, Yunfeng
中科院分区：
文献类型：
--
作者：
An, Diaoguo;Zheng, Qi;Xu, Yunfeng
黑麦是小麦改良的重要基因资源。然而，由于黑麦1 R短臂抗病基因的广泛种植以及病原菌毒力与寄主抗性的协同进化，这些品种相继丧失了对病原菌的抗性。因此，鉴定和利用黑麦新的抗病基因资源具有特别重要和紧迫的意义。以普通小麦品种小偃6号和黑麦品种德国白色为材料，通过远缘杂交和染色体工程技术，获得了小麦黑麦新品系WR 41 -1。经序列基因组原位杂交（GISH）、荧光原位杂交（mc-FISH）和表达序列标签-简单重复序列（EST-SSR）标记分析，证明该易位系是一个新的小麦-黑麦T4 BL center dot 4 RL和T7 AS center dot 4 RS易位系。WR 41 -1对小麦白粉病（Blumeria graminis f. 23个菌株中有13个在苗期进行了检测。根据WR 41 - 1对23个不同的Bgt菌株的抗性模式，WR 41 -1可能具有新的抗白粉病基因，这些基因不同于已鉴定和已知的黑麦白粉病基因（Pm 7、Pm 8、Pm 17和Pm20）。此外，WR 41 -1细胞学稳定，育性好，有望在小麦改良中发挥重要作用。
Rye is an important and valuable gene resource for wheat improvement. However, due to extensive growing of cultivars with disease resistance genes from short arm of rye chromosome 1R and coevolution of pathogen virulence and host resistance, these cultivars successively lost resistance to pathogens. Identification and deployment of new resistance gene sources in rye are, therefore, of especial importance and urgency. A new wheat-rye line, designated as WR41-1, was produced through distant hybridization and chromosome engineering protocols between common wheat cultivar Xiaoyan 6 and rye cultivar German White. It was proved to be a new wheat-rye T4BL center dot 4RL and T7AS center dot 4RS translocation line using sequential genomic in situ hybridization (GISH), multicolor fluorescence in situ hybridization (mc-FISH), and expressed sequence tag-simple sequence repeat (EST-SSR) marker analysis. WR41-1 showed high levels of resistance to powdery mildew (Blumeria graminis f. sp. tritici, Bgt) pathogens prevalent in China at the adult growth stage and 13 of 23 Bgt isolates tested at the seedling stage. According to its resistant pattern to 23 different Bgt isolates, WR41-1 may possess new gene(s) for resistance to powdery mildew, which differed from previously identified and known powdery mildew genes from rye (Pm7, Pm8, Pm17, and Pm20). In addition, WR41-1 was cytologically stable, had a desirable fertility, and is expected to be useful in wheat improvement.