Chromosomal Position Mediates Spinal Cord Expression of a dbx1a Enhancer
Chromosomal Position Mediates Spinal Cord Expression of a dbx1a Enhancer
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DOI：
10.1002/dvdy.22122
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发表时间：
2009-11-01
影响因子：
2.5
通讯作者：
Dorsky, Richard I.
中科院分区：
文献类型：
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作者：
Gribble, Suzanna L.;Nikolaus, O. Brant;Dorsky, Richard I.
Dbx同源结构域蛋白对多种脊髓细胞类型的产生很重要。为了更详细地研究Dbx基因的调控，我们培育了转基因斑马鱼，其中荧光蛋白的表达由预测的dbx1a增强子驱动。我们确定了dbx1a编码序列上游的三个序列保守区域，并由这些预测的增强子元件和内源性dbx1a启动子驱动生成了荧光报告结构。在3.5 kb增强子片段的多次稳定插入中，我们观察到在脊髓背侧有额外的报告基因表达，这是dbx1a原位杂交通常观察不到的。此外，与内源基因不同，这些系只表现出短暂的报告基因表达。令人惊讶的是，单个插入线以内源性模式表达报告子，表明其他局部调控元件通过3.5 kb增强子调节基因表达。科学通报，2009(4):379 - 379。(C) 2009 Wiley-Liss, Inc。
Dbx homeodomain proteins are important for the production of multiple spinal cord cell types. To examine the regulation of Dbx genes in more detail, we have generated transgenic zebrafish in which fluorescent protein expression is driven by predicted dbx1a enhancers. We identified three areas of sequence conservation upstream of the dbx1a coding sequence and generated fluorescent reporter constructs driven by these predicted enhancer elements and the endogenous dbx1a promoter. In multiple stable insertions of a 3.5-kb enhancer fragment, we observed that there was additional reporter expression in the dorsal spinal cord not normally observed by dbx1a in situ hybridization. In addition, these lines exhibited only transient reporter expression, unlike the endogenous gene. Surprisingly, a single insertion line expressed the reporter in the endogenous pattern, indicating that other local regulatory elements modulate gene expression through the 3.5-kb enhancer. Developmental Dynamics 238:2929-2935, 2009. (C) 2009 Wiley-Liss, Inc.