Feasibility of self-collection of fecal specimens by randomly sampled women for health-related studies of the gut microbiome.
Feasibility of self-collection of fecal specimens by randomly sampled women for health-related studies of the gut microbiome.
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DOI：
10.1186/1756-0500-7-204
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发表时间：
2014-04-01
影响因子：
1.8
通讯作者：
Goedert, James J
中科院分区：
文献类型：
--
作者：
Feigelson, Heather Spencer;Bischoff, Kimberly;Goedert, James J
背景：微生物组研究领域正在迅速发展。我们开发了一种自行采集粪便标本的方法，可用于基于人群的肠道微生物组研究。我们进行了一项试点研究，以测试我们的方法的可行性，在健康，绝经后妇女谁是凯撒永久科罗拉多（KPCO）的成员随机抽样。我们的目的是收集问卷调查数据，粪便和尿液标本，从60名妇女，年龄55-69，谁最近有一个正常的筛查乳房X光检查。我们设计的研究，所有的问卷数据和标本可以收集在home.RESULTS：我们邮寄邀请包，同意书和选择退出明信片300名妇女，然后招募电话妇女谁没有选择退出。口头同意的女性被邮寄入组包，包括风险因素问卷，链接到在线饮食问卷，标本收集试剂盒，并收集粪便和尿液的说明。标本连夜运送到生物储存库。在300名邮寄了邀请函的女性中，有58人（19%）退回了选择退出的明信片。对其余妇女进行了多达3次电话联系，其中130人（43%）无法联系，23人（8%）被拒绝，12人（4%）不合格。登记包邮寄给77名妇女，其中59人返回的危险因素问卷和标本。我们发现，没有统计学上的显着差异，登记的妇女和那些谁拒绝参与或无法联系。结论：我们证明，一个有代表性的样本的妇女可以成功地招募肠道微生物组研究，但是，重要的个人接触和仔细定时的后续研究人员是必需的。我们的研究所采用的方法可以成功地应用于广泛的临床条件的分析研究，这些条件被认为是受肠道微生物群的影响。
BACKGROUND: The field of microbiome research is growing rapidly. We developed a method for self-collection of fecal specimens that can be used in population-based studies of the gut microbiome. We conducted a pilot study to test the feasibility of our methods among a random sample of healthy, postmenopausal women who are members of Kaiser Permanente Colorado (KPCO). We aimed to collect questionnaire data, fecal and urine specimens from 60 women, aged 55-69, who recently had a normal screening mammogram. We designed the study such that all questionnaire data and specimens could be collected at home.RESULTS: We mailed an invitation packet, consent form and opt-out postcard to 300 women, then recruited by telephone women who did not opt-out. Verbally consented women were mailed an enrollment package including a risk factor questionnaire, link to an online diet questionnaire, specimen collection kit, and instructions for collecting stool and urine. Specimens were shipped overnight to the biorepository. Of the 300 women mailed an invitation packet, 58 (19%) returned the opt-out postcard. Up to 3 attempts were made to telephone the remaining women, of whom 130 (43%) could not be contacted, 23 (8%) refused, and 12 (4%) were ineligible. Enrollment packages were mailed to 77 women, of whom 59 returned the risk factor questionnaire and specimens. We found no statistically significant differences between enrolled women and those who refused participation or could not be contacted.CONCLUSIONS: We demonstrated that a representative sample of women can be successfully recruited for a gut microbiome study; however, significant personal contact and carefully timed follow-up from the study personnel are required. The methods employed by our study could successfully be applied to analytic studies of a wide range of clinical conditions that have been postulated to be influenced by the gut microbial population.