Downregulation of paraoxonase 3 contributes to aggressive human hepatocellular carcinoma progression and associates with poor prognosis.
Downregulation of paraoxonase 3 contributes to aggressive human hepatocellular carcinoma progression and associates with poor prognosis.
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对氧磷酶 3 的下调有助于侵袭性人类肝细胞癌的进展，并与不良预后相关。
DOI：
10.1007/s13277-016-5247-z
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Ye Le-Chi
中科院分区：
文献类型：
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作者：
Jin Yuepeng;Li Qiang;Qiu Junjun;Zhao Xiufen;Zheng Chunxiao;Lv Shixu;Bai Yongyu;Shan Yunfeng;Ye Le-Chi
对氧磷酶（PON）具有抗氧化特性，可预防心血管疾病。作为PON家族的一员，PON 3主要在肝脏中合成，研究较少。本研究旨在检测PON 3在人肝细胞癌（HCC）中的表达，探讨PON 3在HCC患者中的临床意义和生物学功能。我们首先使用免疫组织化学（IHC）分析了50对HCC样本（HCC组织与匹配的癌旁组织）和160例临床HCC标本中的PON 3表达。我们的研究结果表明，PON 3在HCC中的表达下调，并与肿瘤淋巴结转移（TNM）分期、肿瘤大小和肿瘤数量显著相关。Kaplan-Meier生存分析和考克斯回归分析显示PON 3是影响总生存期（OS）和复发时间（TTR）的独立预后因素。最后，我们的目的是揭示PON 3在肝癌生长和转移中的生物学功能，我们的结果表明，PON 3的过表达有力地抑制肝癌的生长和转移。总的来说，我们的研究表明，PON 3表现出对肝癌的肿瘤抑制作用，它可能作为一种新的预后指标在肝癌。
Paraoxonase (PON) enzymes possess antioxidant properties and protect against cardiovascular diseases. As a member of PON family, PON3 is primarily synthesized in the liver and poorly investigated. This study aimed to examine the expression of PON3 in human hepatocellular carcinoma (HCC) and investigate the clinical significance and biological function of PON3 in HCC patients. We first analyzed PON3 expression in 50 paired HCC samples (HCC tissues vs matched para-cancerous tissues) and 160 clinical HCC specimens by using immunohistochemistry (IHC). Our results showed that the expression of PON3 was downregulated in HCC and significantly associated with tumor-node-metastasis (TNM) stage, tumor size, and tumor number. Kaplan-Meier survival and Cox regression analyses showed that PON3 was an independent prognostic factor for overall survival (OS) and time to recurrence (TTR). Finally, we aimed to reveal the biological function of PON3 in HCC growth and metastasis, and our results showed that overexpression of PON3 potently inhibited growth and metastasis of HCC. Collectively, our study demonstrated that PON3 exhibited tumor-suppressive effects toward HCC and it might serve as a novel prognostic marker in HCC.