Fat Mass and Obesity-Associated Gene Enhances Oxidative Stress and Lipogenesis in Nonalcoholic Fatty Liver Disease
Fat Mass and Obesity-Associated Gene Enhances Oxidative Stress and Lipogenesis in Nonalcoholic Fatty Liver Disease
复制标题
脂肪量和肥胖相关基因增强非酒精性脂肪肝的氧化应激和脂肪生成
DOI：
10.1007/s10620-012-2516-6
复制
发表时间：
2013-04-01
影响因子：
3.1
通讯作者：
Jia, Weiping
中科院分区：
文献类型：
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作者：
Guo, Jianjin;Ren, Wei;Jia, Weiping
背景和目的非酒精性脂肪性肝病（NAFLD）与肥胖、高脂血症和2型糖尿病密切相关。几项研究发现，脂肪量和肥胖相关基因（FTO）与肥胖有关。本研究旨在探讨FTO在脂质代谢疾病肝脏中的表达和功能。方法观察NAFLD大鼠模型中FTO的基础表达，并与对照组进行比较。通过过表达实验进一步研究FTO在L02细胞脂质代谢中的作用。结果NAFLD组FTO mRNA和蛋白水平显著升高。此外，FTO水平与丙二醛和超氧化物歧化酶浓度呈正相关。FTO在L02细胞中的过表达增强了脂肪生成和氧化应激。结论肝脏FTO水平升高与NAFLD的氧化应激和脂质沉积有关。
Background and AimNonalcoholic fatty liver disease (NAFLD) is strongly associated with obesity, hyperlipidemia, and type 2 diabetes mellitus. Several studies have found that fat mass and the obesity-associated (FTO) gene is linked to obesity. The aim of this work is to investigate the expression and function of FTO in liver with lipid metabolism diseases.MethodsWe investigated the basal FTO expression in an NAFLD rat model and compared it with control subjects. The function of FTO in lipid metabolism was further studied in L02 cells through overexpression experiments.ResultsA significant increase in FTO mRNA and protein levels was found in the NAFLD group. In addition, the FTO levels were positively associated with malondialdehyde and superoxide dismutase concentrations. FTO overexpression in L02 cells enhanced lipogenesis and oxidative stress.ConclusionsThis study demonstrates that increased FTO levels in the liver are involved in oxidative stress and lipid deposition, which characterize NAFLD.