Engineering ligand-responsive gene-control elements: lessons learned from natural riboswitches.
Engineering ligand-responsive gene-control elements: lessons learned from natural riboswitches.
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DOI：
10.1038/gt.2009.81
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发表时间：
2009-10
期刊：
影响因子：
5.1
通讯作者：
Breaker RR
中科院分区：
文献类型：
--
作者：
Link KH;Breaker RR
In the past two decades, remarkable advances have been made in the development of technologies used to engineer new aptamers and ribozymes. This has encouraged interest among researchers who seek to create new types of gene control systems that can be made to respond specifically to small molecule signals. Validation that RNA molecules can exhibit the characteristics needed to serve as precision genetic switches has come from the discovery of numerous classes of natural ligand-sensing RNAs called riboswitches. While a great deal of progress has been made towards engineering useful designer riboswitches, considerable advances are needed before the performance characteristics of these RNAs match those of protein systems that have been co-opted to regulate gene expression. In this review, we will evaluate the potential for engineered RNAs to regulate gene expression and lay out possible paths to designer riboswitches based upon currently available technologies. Furthermore, we will discuss some technical advances that would empower RNA engineers who seek to make routine the production of designer riboswitches that can function in eukaryotes.
