Regional control of histone H3 lysine 27 methylation in Neurospora
Regional control of histone H3 lysine 27 methylation in Neurospora
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DOI：
10.1073/pnas.1303750110
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发表时间：
2013-04-09
影响因子：
11.1
通讯作者：
Selker, Eric U.
中科院分区：
文献类型：
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作者：
Jamieson, Kirsty;Rountree, Michael R.;Selker, Eric U.
组蛋白H3上的三甲基化赖氨酸27（H3 K27 me 3）存在于果蝇、拟南芥、蠕虫和哺乳动物中，但在已检测的酵母中不存在。我们鉴定并分析了丝状真菌粗糙脉孢菌和其他脉孢菌属物种中的H3 K27 me 3。H3 K27 me 3占N的6.8%。crassa基因组，包含223个结构域，包括774个基因，所有这些都是转录沉默的。N. crassa中H3 K27 me 3标记基因的保守性低于未标记基因，仅与N. crassa在离散脉孢菌（Neurospora discreta）和四孢脉孢菌（Neurospora tetrasperma）中也是H3 K27 me 3标记的。我们发现H3 K27 me 3需要链孢菌多梳抑制复合物2（PRC 2）的三个组分[Su-（var）3-9; E（z）; Trithorax]（SET）-7、胚胎外胚层发育（EED）和SU（Z）12（zeste 12的抑制子），而第四个组分链孢菌蛋白55（一个N. p55/RbAp 48的crassa同源物），仅在亚端粒结构域对H3 K27 me 3至关重要。H3 K27 me 3的缺失，由编码催化PRC 2亚基set-7的基因的缺失引起，导致130个基因的上调，包括H3 K27 me 3标记和未标记区域的基因。
Trimethylated lysine 27 on histone H3 (H3K27me3) is present in Drosophila, Arabidopsis, worms, and mammals, but is absent from yeasts that have been examined. We identified and analyzed H3K27me3 in the filamentous fungus Neurospora crassa and in other Neurospora species. H3K27me3 covers 6.8% of the N. crassa genome, encompassing 223 domains, including 774 genes, all of which are transcriptionally silent. N. crassa H3K27me3-marked genes, are less conserved than unmarked genes and only similar to 35% of genes marked by H3K27me3 in N. crassa are also H3K27me3-marked in Neurospora discreta and Neurospora tetrasperma. We found that three components of the Neurospora Polycomb repressive complex 2 (PRC2)-[Su-(var)3-9; E(z); Trithorax] (SET)-7, embryonic ectoderm development (EED), and SU(Z)12 (suppressor of zeste12)-are required for H3K27me3, whereas the fourth component, Neurospora protein 55 (an N. crassa homolog of p55/RbAp48), is critical for H3K27me3 only at subtelomeric domains. Loss of H3K27me3, caused by deletion of the gene encoding the catalytic PRC2 subunit, set-7, resulted in up-regulation of 130 genes, including genes in both H3K27me3-marked and unmarked regions.