Relative importance of surface wettability and charged functional groups on NIH 3T3 fibroblast attachment, spreading, and cytoskeletal organization
Relative importance of surface wettability and charged functional groups on NIH 3T3 fibroblast attachment, spreading, and cytoskeletal organization
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DOI：
10.1002/(sici)1097-4636(19980905)41:3
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发表时间：
1998-09-05
期刊：
影响因子：
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通讯作者：
Tresco, PA
中科院分区：
文献类型：
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作者：
Webb, K;Hlady, V;Tresco, PA
了解材料表面性质、吸附蛋白质和细胞反应之间的关系对于设计用于植入和组织工程的最佳材料表面至关重要。在这项研究中，我们已经准备了不同的功能基团的模型表面，以提供一系列的表面润湿性和电荷。附着，传播和细胞骨架组织的细胞反应进行了研究，这些表面与稀释血清，特定的血清蛋白，和细胞外基质的个别成分的预吸附。当预吸附稀释血清，细胞附着，传播，和细胞骨架组织的亲水表面相对于疏水表面显着更大。在亲水性表面中，电荷和润湿性的差异影响细胞附着，但不影响细胞面积、形状或细胞骨架组织。适度亲水的表面（20-40度水接触角）促进最高水平的细胞附着。预吸附的模型表面与牛血清白蛋白（BSA）的细胞附着的模式非常相似，观察到以下预吸附与稀释的血清，这表明BSA在调节细胞附着的生物材料暴露于复杂的生物介质中的重要作用。(C)John Wiley & Sons，Inc.
Understanding the relationships between material surface properties, adsorbed proteins, and cellular responses is essential to designing optimal material surfaces for implantation and tissue engineering. In this study, we have prepared model surfaces with different functional groups to provide a range of surface wettability and charge. The cellular responses of attachment, spreading, and cytoskeletal organization have been studied following preadsorption of these surfaces with dilute serum, specific serum proteins, and individual components of the extracellular matrix. When preadsorbed with dilute serum, cell attachment, spreading, and cytoskeletal organization were significantly greater on hydrophilic surfaces relative to hydrophobic surfaces. Among the hydrophilic surfaces, differences in charge and wettability influenced cell attachment but not cell area, shape, or cytoskeletal organization. Moderately hydrophilic surfaces (20-40 degree water contact angle) promoted the highest levels of cell attachment. Preadsorption of the model surfaces with bovine serum albumin (BSA) resulted in a pattern of cell attachment very similar to that observed following preadsorption with dilute serum, suggesting an important role for BSA in regulating cell attachment to biomaterials exposed to complex biological media. (C) 1998 John Wiley & Sons, Inc.