Role of the leader sequence in tobacco pectin methylesterase secretion
Role of the leader sequence in tobacco pectin methylesterase secretion
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DOI：
10.1016/j.febslet.2006.04.090
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发表时间：
2006-05-29
期刊：
影响因子：
3.5
通讯作者：
Atabekov, Joseph G.
中科院分区：
文献类型：
--
作者：
Dorokhov, Yuri L.;Skurat, Eugene V.;Atabekov, Joseph G.
我们报道了未加工的烟草果胶甲基酯酶(PME)含有N末端的前序列，包括跨膜(TM)结构域和成熟PME之前的间隔区。用绿色荧光蛋白(GFP)基因取代PME的成熟部分，并将其与GFP融合的前序列的各种缺失突变体克隆到双元载体中，并将其农业注射到本氏烟草叶片中。PME前序列将GFP运送到细胞壁(CW)。我们发现，PME TM结构域在转运到CW时与内质网膜发生瞬时结合。TM域的各种缺失取消了CW的靶向性，即锚定域是GFP分泌到CW所必需的。相反，即使整个间隔区的缺失也不会对GFP靶向产生影响。(C)2006年欧洲生化学会联合会。爱思唯尔出版，版权所有。
We report that unprocessed tobacco pectin methylesterase (PME) contains N-terminal pro-sequence including the transmembrane (TM) domain and spacer segment preceding the mature PME. The mature portion of PME was replaced by green fluorescent protein (GFP) gene and various deletion mutants of pro-sequence fused to GFP were cloned into binary vectors and agroinjected in Nicotiana benthamiana leaves. The PME pro-sequence delivered GFP to the cell wall (CW). We showed that a transient binding of PME TM domain to endoplasmic reticulum membranes occurs upon its transport to CW. The CW targeting was abolished by various deletions in the TM domain, i.e., anchor domain was essential for secretion of GFP to CW. By contrast, even entire deletion of the spacer segment had no influence on GFP targeting. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.