Implementing the CURE: Combining Wet‐Lab Protein Biochemistry with Computational Analysis to Provide Gains in Student Learning in the Biochemistry Teaching Lab
Implementing the CURE: Combining Wet‐Lab Protein Biochemistry with Computational Analysis to Provide Gains in Student Learning in the Biochemistry Teaching Lab
复制标题
实施 CURE：将湿实验室蛋白质生物化学与计算分析相结合，为学生在生物化学教学实验室中的学习提供收益
DOI：
10.1096/fasebj.2018.32.1_supplement.663.21
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Pikaart, Michael
中科院分区：
文献类型：
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作者：
Pikaart, Michael
大多数学习生物化学和分子生物学的本科生在他们的高级实验室课程中最广泛地接触蛋白质和核酸化学（以及越来越多的计算机/可视化）的湿实验室技术。这些人往往是有明确职业目标的大三和大四学生。其中一些学生已经在传统的一对一（或一对几）研究指导环境中具有研究背景，例如夏季研究计划。事实证明，这种方法在提高学生对科学的学习和坚持方面是有效的。与此同时，延长全日制PI指导的研究是有限的学生人数，甚至可以提出一个障碍。为了通过研究扩大教学的影响，许多从业者采用了CURE，或基于课程的本科生研究经验，方法。本报告报告介绍了“BASIL”（生物化学真实科学探究实验室），一个致力于将计算和湿实验室蛋白质科学引入生物化学教学实验室的教师团队。我们共同开发了一种蛋白质生物化学CURE，用于确定未知活性蛋白质的酶功能。这项工作利用了蛋白质结构计划的结果，该计划是NIH资助的一项为期15年的工作，于2015年结束，出版和分发了5000多种以前未表征的蛋白质。其中绝大多数是“孤儿”，具有高质量的结构和预克隆的表达质粒，但没有研究其酶功能或在天然生物中的作用。本科生生物化学教师和学生的BASIL联盟旨在确定这些未表征蛋白质的子集的功能特性，寻求统一的结构和功能关系。目前，可实施的模块可供教师谁愿意采用它们，并预期的学生成绩将被提交。支持或资助信息NSF IUSE 1709278支持此摘要来自实验生物学2018会议。在FASEB期刊上没有与此摘要相关的全文文章。
Most undergraduates studying biochemistry and molecular biology get their broadest exposure to wet-lab techniques in protein and nucleic acid chemistry (and, increasingly, computer/visualization) in their upper-level laboratory courses. These tend to be juniors and seniors with well-defined career goals. Some of these students will have already have a research background in a traditional one-to-one (or one-to-few) research mentoring setting, for example a summer research program. This approach has proved effective at increasing student learning and persistence in the sciences. At the same time, extended full-time PI-directed research is limited in the number of students served, and can even present a barrier. To broaden the impact of teaching through research, many practitioners have adopted a CURE, or Course-based Undergraduate Research Experience, approach.This presentation reports on “BASIL” (Biochemical Authentic Scientific Inquiry Laboratory), a team of faculty who have worked to bring computational and wet-lab protein science to the biochemistry teaching lab. Together, we have developed a protein biochemistry CURE to determine enzymatic function of proteins of unknown activity. This work leverages the results of the Protein Structure Initiative, a fifteen-year NIH-funded effort which concluded in 2015 with the publication and distribution of more than 5000 previously uncharacterized proteins. The great majority of these are “orphans,” with high quality structures and pre-cloned expression plasmids available, but no research on their enzymatic function or role in native organisms. The BASIL consortium of undergraduate biochemistry faculty and students seeks to identify functional properties of a subset of these uncharacterized proteins, seeking to unify structure and function relationships. Currently, implementable modules are available for faculty who wish to adopt them, and expected student results will be presented.Support or Funding InformationSupported by NSF IUSE 1709278This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.