Transcription factors perform a 2-step search of the nucleus
Transcription factors perform a 2-step search of the nucleus
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转录因子对细胞核进行两步搜索
DOI：
10.1093/genetics/iyac111
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发表时间：
2022
期刊：
影响因子：
3.3
通讯作者：
Rine, ed., J.
中科院分区：
文献类型：
--
作者：
Staller, Max Valentín;Rine, ed., J.
转录因子通过与调控DNA结合并募集调控蛋白复合物来调控基因表达。转录因子的DNA结合和蛋白质结合功能传统上被描述为由模块化蛋白质结构域执行的独立功能。在这里，我认为，基因组结合可以是一个2部分的过程，既有DNA结合和蛋白质结合的步骤，使转录因子进行两步搜索的细胞核中找到合适的结合位点在真核基因组。我支持这一假设与新的和旧的文献中的结果，讨论这一假设如何吝啬地解决悬而未决的问题，并提出可检验的预测。
Transcription factors regulate gene expression by binding to regulatory DNA and recruiting regulatory protein complexes. The DNA-binding and protein-binding functions of transcription factors are traditionally described as independent functions performed by modular protein domains. Here, I argue that genome binding can be a 2-part process with both DNA-binding and protein-binding steps, enabling transcription factors to perform a 2-step search of the nucleus to find their appropriate binding sites in a eukaryotic genome. I support this hypothesis with new and old results in the literature, discuss how this hypothesis parsimoniously resolves outstanding problems, and present testable predictions.