NITRIC-OXIDE SYNTHASE INHIBITORS ATTENUATE HUMAN MONOCYTE CHEMOTAXIS INVITRO
NITRIC-OXIDE SYNTHASE INHIBITORS ATTENUATE HUMAN MONOCYTE CHEMOTAXIS INVITRO
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DOI：
10.1002/jlb.53.5.498
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发表时间：
1993-05-01
影响因子：
5.5
通讯作者：
RUBINSTEIN, I
中科院分区：
文献类型：
--
作者：
BELENKY, SN;ROBBINS, RA;RUBINSTEIN, I
一氧化氮合酶（NOS）抑制剂已被证明可以调节中性粒细胞迁移。我们假设NOS抑制剂N（G）-单甲基-L-精氨酸（L-NMMA）、N（G）-硝基-L-精氨酸甲酯（L-NAME）和L-刀豆氨酸（L-CAN）也调节人外周血单核细胞趋化性。为了验证这一假设，单核细胞趋化性对甲酰甲硫氨酰-亮氨酰-苯丙氨酸（fMLP）进行了评估，使用改良的Blindwell趋化室技术。L-NMMA和L-NAME，而不是D-NMMA或L-CAN，显著减弱fMLP诱导的单核细胞趋化性（P <0.05）。L-精氨酸和硝普钠，但不是D-精氨酸，逆转NOS神经元诱导的反应。环磷酸鸟苷（cGMP）可减弱L-NMMA对单核细胞趋化性的抑制作用（P <0.05）。最后，fMLP增加单核细胞产生cGMP，这被L-NMMA显著减弱（P <0.05）。这些数据表明L-精氨酸/NO生物合成途径在体外调节人类单核细胞趋化性。
Nitric oxide synthase (NOS) inhibitors have been shown to modulate neutrophil migration. We hypothesized that the NOS inhibitors N(G)-monomethyl-L-arginine (L-NMMA), N(G)-nitro-L-arginine methyl ester (L-NAME), and L-canavanine (L-CAN) also modulate human peripheral blood monocyte chemotaxis. To test this hypothesis, monocyte chemotaxis toward formylmethionyl-leucyl-phenylalanine (fMLP) was assessed using a modified blindwell chemotaxis chamber technique. L-NMMA and L-NAME, but not D-NMMA or L-CAN, significantly attenuated fMLP-induced monocyte chemotaxis (P < .05). L-Arginine and sodium nitroprusside, but not D-arginine, reversed NOS inhibitor-induced responses. Dibutryl cyclic guanyl monophosphate (cGMP) attenuated the inhibitory effects of L-NMMA on monocyte chemotaxis (P < .05). Finally, fMLP increased cGMP generation by monocytes, which was significantly attenuated by L-NMMA (P < .05). These data indicate that the L-arginine/NO biosynthetic pathway regulates human monocyte chemotaxis in vitro.