APLYSIA CREB2 REPRESSES LONG-TERM FACILITATION - RELIEF OF REPRESSION CONVERTS TRANSIENT FACILITATION INTO LONG-TERM FUNCTIONAL AND STRUCTURAL-CHANGE
APLYSIA CREB2 REPRESSES LONG-TERM FACILITATION - RELIEF OF REPRESSION CONVERTS TRANSIENT FACILITATION INTO LONG-TERM FUNCTIONAL AND STRUCTURAL-CHANGE
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DOI：
10.1016/0092-8674(95)90213-9
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发表时间：
1995-12-15
期刊：
影响因子：
64.5
通讯作者：
KANDEL, ER
中科院分区：
文献类型：
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作者：
BARTSCH, D;GHIRARDI, M;KANDEL, ER
在澳大利亚，行为致敏诱导的从短期到长期促进的转变涉及creb样蛋白，以及立即早期基因ApC/EBP。利用双杂交系统中ApC/EBP的bZIP结构域，我们克隆了ApCREB2，一个在感觉神经元中组成表达的转录因子，类似于人类的CREB2和小鼠的ATF4。在F9细胞中，ApCREB2抑制apcreb -1介导的转录。将抗apcreb2抗体注射到海兔的感觉神经元中，会引起单脉冲5-羟色胺（5-HT），它只会引起持续几分钟的短期促进，但会引起持续1天以上的促进。这种促进具有长期促进的特性：它需要转录和翻译，诱导新的突触连接的生长，并阻断5-羟色胺的5次脉冲进一步促进。
The switch from short- to long-term facilitation induced by behavioral sensitization in Aplysia involves CREB-like proteins, as well as the immediate-early gene ApC/EBP. Using the bZIP domain of ApC/EBP in a two-hybrid system, we have cloned ApCREB2, a transcription factor constitutively expressed in sensory neurons that resembles human CREB2 and mouse ATF4. ApCREB2 represses ApCREB-1-mediated transcription in F9 cells. Injection of anti-ApCREB2 antibodies into Aplysia sensory neurons causes a single pulse of serotonin (5-HT), which induces only short-term facilitation lasting minutes, to evoke facilitation lasting more than 1 day. This facilitation has the properties of long-term facilitation: it requires transcription and translation, induces the growth of new synaptic connections, and occludes further facilitation by five pulses of 5-HT.