Molecular correlates of sleep and wakefulness in the brain of the white-crowned sparrow
Molecular correlates of sleep and wakefulness in the brain of the white-crowned sparrow
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DOI：
10.1111/j.1471-4159.2007.05089.x
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发表时间：
2008-04-01
影响因子：
4.7
通讯作者：
Cirelli, Chiara
中科院分区：
文献类型：
--
作者：
Jones, Stephany;Pfister-Genskow, Martha;Cirelli, Chiara
在哺乳动物的大脑中，睡眠和清醒与基因表达的广泛变化有关。然而，警惕状态的分子相关因子在整个系统发育中保守的程度才刚刚开始被探索。这项研究的目的是确定睡眠和清醒是否会影响鸟类大脑中的基因表达。为了达到这一目的，我们对白冠麻雀（Zonotrichia leucophrys gambelii）在睡眠、清醒和短期睡眠剥夺期间的端脑基因表达进行了广泛的微阵列分析。我们发现，在啮齿类动物的大脑皮层中，行为状态与一天中的时间无关，对鸟类大脑基因表达有广泛的影响，影响255个基因的转录水平（占所有测试转录的1.4%）。觉醒相关转录本（n = 114）编码参与能量代谢和氧化磷酸化的蛋白质，与活动依赖性神经可塑性相关的即时早期基因和转录因子，以及与未折叠蛋白质反应相关的热休克蛋白和分子伴侣蛋白。睡眠相关转录本（n = 141）编码涉及膜运输、脂质/胆固醇合成、翻译调节、细胞粘附和细胞骨架组织的蛋白质。值得注意的是，尽管哺乳动物新皮层和鸟类端脑在形态学和细胞学上存在相当大的差异，但本研究中鉴定的转录本的功能类别与在啮齿动物皮层中鉴定的转录本有很大程度的重叠。
In the mammalian brain, sleep and wakefulness are associated with widespread changes in gene expression. The extent to which the molecular correlates of vigilance state are conserved across phylogeny, however, is only beginning to be explored. The goal of this study was to determine whether sleep and wakefulness affect gene expression in the avian brain. To achieve this end we performed an extensive microarray analysis of gene expression during sleep, wakefulness, and short-term sleep deprivation in the telencephalon of the white-crowned sparrow (Zonotrichia leucophrys gambelii). We found that, as in the rodent cerebral cortex, behavioral state, independent of time of day, has widespread effects on avian brain gene expression, affecting the transcript levels of 255 genes (1.4% of all tested transcripts). Wakefulness-related transcripts (n = 114) code for proteins involved in energy metabolism and oxidative phosphorylation, immediate early genes and transcription factors associated with activity-dependent neural plasticity, as well as heat-shock proteins and molecular chaperones associated with the unfolded protein response. Sleep-related transcripts (n = 141) code for proteins involved in membrane trafficking, lipid/cholesterol synthesis, translational regulation, cellular adhesion, and cytoskeletal organization. Remarkably, despite the considerable differences in morphology and cytology between the mammalian neocortex and the avian telencephalon, the functional categories of transcripts identified in this study exhibit a significant degree of overlap with those identified in the rodent cortex.