Use of the BACTEC mycobacteria growth indicator tube 960 automated system for recovery of mycobacteria from 9,558 extrapulmonary specimens, including urine samples
Use of the BACTEC mycobacteria growth indicator tube 960 automated system for recovery of mycobacteria from 9,558 extrapulmonary specimens, including urine samples
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DOI：
10.1128/jcm.00829-06
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发表时间：
2006-11-01
影响因子：
9.4
通讯作者：
Ruesch-Gerdes, Sabine
中科院分区：
文献类型：
--
作者：
Hillemann, Doris;Richter, Elvira;Ruesch-Gerdes, Sabine
应用BACTEC分枝杆菌生长指示管960 （MGIT 960）系统从肺外标本中回收分枝杆菌，并与固体培养基（Lowenstein-Jensen和Stone-brink）进行比较。总共调查了9558份标本，包括3074份体液、1878份组织和2069份尿液样本，其中MGIT 960分枝杆菌的回收尚未确定。总体而言，MGIT 960能够检测出494株结核分枝杆菌复合体（MTBC）中的446株（90.3%）和259株非结核分枝杆菌（NTM）中的223株（86.0%）。与此相比，在固体培养基上培养出358株（72.6%）MTBC和164株（66.8%）NTM。MTBC分离株136株（27.6%）和NTM分离株95株（19.2%）仅从MGIT 960培养基中分离得到，而MTBC分离株48株（9.7%）和NTM分离株36株（13.9%）仅在固体培养基中生长。因此，MGIT 960和固体培养基对肺外标本中分枝杆菌的总体敏感性分别为88.8%和69.3%。然而，MGIT 960系统的效率可以通过在固体培养基上添加额外的培养物来最大化。
The BACTEC Mycobacteria Growth Indicator Tube 960 (MGIT 960) system was applied for recovery of mycobacteria from extrapulmonary specimens and compared with solid media (Lowenstein-Jensen and Stone-brink). A total of 9,558 specimens were investigated, comprising 3,074 body fluids, 1,878 tissues, and 2,069 urine samples, from which the recovery of mycobacteria was not yet established for MGIT 960. In total, the MGIT 960 was able to detect 446 (90.3%) of the 494 isolates of Mycobacterium tuberculosis complex (MTBC) and 223 (86.0%) out of the 259 isolates of nontuberculous mycobacteria (NTM). In comparison to this, culture on solid medium revealed 358 (72.6%) MTBC isolates and 164 (66.8%) NTM isolates. While 136 (27.6%) of the MTBC isolates and 95 (19.2%) of the NTM isolates were recovered from the MGIT 960 only, 48 (9.7%) of the MTBC isolates and 36 (13.9%) NTM isolates grew only on solid media. Thus, the overall sensitivities for the recovery of mycobacteria from extrapulmonary specimens with MGIT 960 and solid media were 88.8% and 69.3%, respectively. However, the efficiency of the MGIT 960 system can be maximized with additional culture on solid media.