N-Heterocyclic Carbene-Gold(I) Complexes Conjugated to a Leukemia-Specific DNA Aptamer for Targeted Drug Delivery.
N-Heterocyclic Carbene-Gold(I) Complexes Conjugated to a Leukemia-Specific DNA Aptamer for Targeted Drug Delivery.
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N-杂环碳烯基（I）复合物与白血病特异性DNA适体共轭，用于靶向药物。
DOI：
10.1002/anie.201602702
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发表时间：
2016-07-25
期刊：
影响因子：
--
通讯作者：
Veige AS
中科院分区：
文献类型：
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作者：
Niu W;Chen X;Tan W;Veige AS
本报告描述了新型N-杂环卡宾（NHC）金（I）配合物的合成和表征及其与CCRF-CEM白血病特异性适体sgc 8 c的生物缀合。通过在NHC-Au（I）复合物和适体上使用荧光标签来实现成功的生物缀合的确认。细胞活力测定表明NHC-Au（I）-适体缀合物比单独的NHC-金络合物更具细胞毒性。流式细胞术、共聚焦显微镜和细胞活力测定的组合提供了明确的证据，表明NHC-Au（I）-适体缀合物对靶向的CCRF-CEM白血病细胞具有选择性。
This report describes the synthesis and characterization of novel N-heterocyclic carbene (NHC) gold(I) complexes and their bioconjugation to the CCRF-CEM leukemia specific aptamer sgc8c. Confirmation of successful bioconjugation was achieved by using fluorescent tags on both the NHC-Au(I) complex and the aptamer. Cell viability assays indicate the NHC-Au(I)-aptamer conjugate is more cytotoxic than the NHC-gold complex alone. A combination of flow cytometry, confocal microscopy, and cell viability assays provide clear evidence that the NHC-Au(I)-aptamer conjugate is selective for targeted CCRF-CEM leukemia cells.