Human CD8+ T cells recognize epitopes of the 28-kDa hemolysin and the 38-kDa antigen of Mycobacterium tuberculosis
Human CD8+ T cells recognize epitopes of the 28-kDa hemolysin and the 38-kDa antigen of Mycobacterium tuberculosis
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DOI：
10.1189/jlb.0403138
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发表时间：
2003-12-01
影响因子：
5.5
通讯作者：
Wizel, B
中科院分区：
文献类型：
--
作者：
Shams, H;Barnes, PF;Wizel, B
由人CD 8 + T细胞识别的结核分枝杆菌抗原是潜在的重要疫苗靶分子。我们使用基于模体的策略筛选M.结核病的预测结合人类白细胞抗原（HLA）-A*0201的肽。我们确定了两个10个氨基酸的肽，引起溶细胞性T淋巴细胞活性和干扰素-γ的生产的CD 8 + T细胞从HLAA*0201+健康结核菌素反应。这些肽衍生自38-kDa抗原和28-kDa溶血素，后者是CD 8 + T细胞的新靶点。我们推测，溶血素可能会改变吞噬体膜周围的细胞内M。结核病，使自己和其他抗原获得进入主要组织相容性复合物I类加工途径。J. Leukoc. 74：1008-1014; 2003.
Mycobacterium tuberculosis antigens that are recognized by human CD8+ T cells are potentially important vaccine target molecules. We used a motif-based strategy to screen selected proteins of M. tuberculosis for peptides predicted to bind to human leukocyte antigen (HLA)-A*0201. We identified two 10 amino acid peptides that elicited cytolytic T lymphocyte activity and interferon-gamma production by CD8+ T cells from HLAA*0201+ healthy tuberculin reactors. These peptides were derived from the 38-kDa antigen and the 28-kDa hemolysin, the latter being a novel target for CD8+ T cells. We speculate that hemolysins may alter the phagosomal membrane surrounding intracellular M. tuberculosis, allowing themselves and other antigens to gain access to the major histocompatibility complex class I processing pathway. J. Leukoc. Biol. 74: 1008-1014; 2003.