mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines.
mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines.
复制标题
DOI：
10.1016/j.dib.2016.02.085
复制
发表时间：
2016-06
期刊：
影响因子：
1.2
通讯作者：
González-Polo RA
中科院分区：
文献类型：
--
作者：
Gómez-Sánchez R;Yakhine-Diop SM;Rodríguez-Arribas M;Bravo-San Pedro JM;Martínez-Chacón G;Uribe-Carretero E;Pinheiro de Castro DC;Pizarro-Estrella E;Fuentes JM;González-Polo RA
我们使用四种不同的细胞系统并分析在该领域广泛使用的标记物，即LC 3，来表征体外自噬的动力学（微管相关蛋白1轻链3;蛋白质从胞质溶胶（LC 3-I）募集到自噬体膜，在那里它被脂化（LC 3-II））和p62/SQSTM 1（衔接蛋白，充当LC 3和遍在蛋白化底物之间的联系），（Klionsky等人，2016）。所提供的数据包括通过蛋白质印迹和内源性免疫荧光实验分析LC 3和p62的蛋白水平，以及通过定量PCR（qPCR）获得的p62 mRNA水平。为了监测这些自噬标志物的周转，并因此测量该途径的通量，将细胞在饥饿条件下和/或用巴弗洛霉素Al（Baf. A1）阻断自噬体与溶酶体的融合。
We characterized the dynamics of autophagy in vitro using four different cell systems and analyzing markers widely used in this field, i.e. LC3 (microtubule-associated protein 1 light chain 3; protein recruited from the cytosol (LC3-I) to the autophagosomal membrane where it is lipidated (LC3-II)) and p62/SQSTM1 (adaptor protein that serves as a link between LC3 and ubiquitinated substrates), (Klionsky et al., 2016). Data provided include analyses of protein levels of LC3 and p62 by Western-blotting and endogenous immunofluorescence experiments, but also p62 mRNA levels obtained by quantitative PCR (qPCR). To monitor the turnover of these autophagy markers and, thus, measure the flux of this pathway, cells were under starvation conditions and/or treated with bafilomycin A1 (Baf. A1) to block fusion of autophagosomes with lysosomes.