A Quantitative Genetic Basis for Leaf Morphology in a Set of Precisely Defined Tomato Introgression Lines
A Quantitative Genetic Basis for Leaf Morphology in a Set of Precisely Defined Tomato Introgression Lines
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DOI：
10.1105/tpc.113.112391
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发表时间：
2013-07-01
期刊：
影响因子：
11.6
通讯作者：
Sinha, Neelima R.
中科院分区：
文献类型：
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作者：
Chitwood, Daniel H.;Kumar, Ravi;Sinha, Neelima R.
渐渗系是将野生番茄的遗传物质渐渗到栽培番茄中的一种材料，已广泛应用于番茄（Solanum lycopersicum）改良。在这里，我们基因型IL人口来自野生沙漠番茄茄pennellii在10000000密度，提供确切的基因内容，每一行窝藏。为了利用这些信息，我们确定IL表型的一套营养性状，从叶的复杂性，形状和大小的细胞性状，如气孔密度和表皮细胞表型。叶片轮廓上的椭圆形傅立叶描述符提供了高度遗传的叶形态的复杂方面的全球分析。我们还证明了小叶大小和叶的复杂性，路面细胞的大小，气孔密度之间的限制，并显示独立的分离性状以前假设是遗传共调节。对IL中先前测量的性状进行的荟萃分析显示，叶片形态和果实糖水平之间存在意想不到的关系，RNA-Seq数据表明这可能归因于果实形态的遗传共调节变化或叶片形状对光合作用的影响。总之，我们的研究结果既提高了一个重要的遗传资源的效用，并证明了一个复杂的，遗传基础的差异，自然种群之间的叶形态。
Introgression lines (ILs), in which genetic material from wild tomato species is introgressed into a domesticated background, have been used extensively in tomato (Solanum lycopersicum) improvement. Here, we genotype an IL population derived from the wild desert tomato Solanum pennellii at ultrahigh density, providing the exact gene content harbored by each line. To take advantage of this information, we determine IL phenotypes for a suite of vegetative traits, ranging from leaf complexity, shape, and size to cellular traits, such as stomatal density and epidermal cell phenotypes. Elliptical Fourier descriptors on leaflet outlines provide a global analysis of highly heritable, intricate aspects of leaf morphology. We also demonstrate constraints between leaflet size and leaf complexity, pavement cell size, and stomatal density and show independent segregation of traits previously assumed to be genetically coregulated. Meta-analysis of previously measured traits in the ILs shows an unexpected relationship between leaf morphology and fruit sugar levels, which RNA-Seq data suggest may be attributable to genetically coregulated changes in fruit morphology or the impact of leaf shape on photosynthesis. Together, our results both improve upon the utility of an important genetic resource and attest to a complex, genetic basis for differences in leaf morphology between natural populations.