ASCORBATE FREE-RADICAL REDUCTASE, A KEY ENZYME OF THE ASCORBIC-ACID SYSTEM
ASCORBATE FREE-RADICAL REDUCTASE, A KEY ENZYME OF THE ASCORBIC-ACID SYSTEM
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DOI：
10.1016/0014-5793(81)80729-6
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发表时间：
1981-01-01
期刊：
影响因子：
3.5
通讯作者：
BORRACCINO, G
中科院分区：
文献类型：
--
作者：
ARRIGONI, O;DIPIERRO, S;BORRACCINO, G
在发现石蒜碱（石蒜科的生物碱）是抗坏血酸（AA）生物合成的强力抑制剂之后，可以确定大量的AA被用于植物细胞代谢[1，2]，类似于动物中发生的情况[3，4]。细胞代谢主要利用抗坏血酸作为电子供体，因此AA经历连续的氧化和还原。氧化产物是抗坏血酸自由基（AFR）和脱氢抗坏血酸（DHA）。它们可以通过两种酶再转化为AA：AFR-还原酶（NADH：AFR氧化还原酶= NADH：单脱氢抗坏血酸氧化还原酶，EC 1.6。5.4）[5-81和DHA-还原酶（GSH：脱氢抗坏血酸氧化还原酶，EC 1.8.（见第5.1段）[9]。在这里，我们报告的数据有关的相对贡献，这两种酶在维持抗坏血酸系统在还原状态。
Following the finding that lycorine (an alkaloid from Amarillidaceae) is a powerful inhibitor of ascorbic acid (AA) biosynthesis, it was possible to establish that large amounts of AA are utilized in plant cell metabolism [1, 2], similarly to what happens in animals [3, 4]. Cell metabolism utilizes ascorbic acid essentially as an electron donor, so that AA undergoes continuous oxidation and reduction. Oxidation products are ascorbic free radical (AFR) and dehydroascorbic acid (DHA). They can be reconverted to AA by two enzymes: AFR-reductase (NADH: AFRoxidoreductase= NADH: monodehydroascorbate oxidoreductase, EC 1.6. 5.4)[5-81 and DHA-reductase (GSH: dehydroascorbate oxidoreductase, EC 1.8. 5.1)[9]. Here we report data concerning the relative contribution of these two enzymes in maintaining the ascorbic acid system in the reduced state.