Integrated Magneto-Electrochemical Sensor for Exosome Analysis.
Integrated Magneto-Electrochemical Sensor for Exosome Analysis.
复制标题
用于外来体分析的集成磁电化学传感器。
DOI：
10.1021/acsnano.5b07584
复制
发表时间：
2016-02-23
期刊：
影响因子：
17.1
通讯作者：
Lee H
中科院分区：
文献类型：
--
作者：
Jeong S;Park J;Pathania D;Castro CM;Weissleder R;Lee H
Extracellular vesicles, including exosomes, are nanoscale vesicles that carry molecular information of parental cells. They are being pursued as biomarkers of cancers that are difficult to detect or serially follow. Here we present a compact sensor technology for rapid, on-site exosome screening. The sensor is based on an integrated magnetic-electrochemical assay: exosomes are immunomagnetically captured from patient samples, and profiled through electrochemical reaction. By combining magnetic enrichment and enzymatic amplification, the approach enables i) highly sensitive, cell-specific exosome detection, and ii) sensor miniaturization and scale-up for high throughput measurements. As a proof-of-concept, we implemented a portable, eight-channel device, and applied it to screen extracellular vesicles in plasma samples from ovarian cancer patients. The sensor allowed for the profiling of multiple protein markers simultaneously within an hour, outperforming conventional methods in assay sensitivity and speed.
