COMPARISON OF KILLING OF GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIA BY PURE SINGLET OXYGEN
COMPARISON OF KILLING OF GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIA BY PURE SINGLET OXYGEN
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DOI：
10.1128/jb.171.4.2188-2194.1989
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发表时间：
1989-04-01
影响因子：
3.2
通讯作者：
HARTMAN, PE
中科院分区：
文献类型：
--
作者：
DAHL, TA;MIDDEN, WR;HARTMAN, PE
发现革兰氏阴性和革兰氏阳性细菌对细胞外产生的纯单线态氧表现出不同的敏感性。鼠伤寒沙门氏菌和大肠杆菌菌株的杀伤曲线表明多击杀伤，而八叠球菌，金黄色葡萄球菌，乳酸链球菌和粪链球菌的曲线表现出单击动力学。色葡萄缺乏几乎所有细胞壁脂多糖外壳的鼠伤寒深粗糙菌株TA 1975表现出一些外源物质的渗透增强，其对单一氧的反应比S.鼠伤寒沙门氏菌野生型菌株，尽管最大杀灭率似乎非常相似。因此，细胞壁的结构在对单氧的敏感性方面起着重要作用。革兰氏阴性细胞壁的外膜脂多糖部分最初保护细菌免受细胞外单线态氧的影响，尽管它也可以作为次级反应产物的来源，这加剧了细胞杀伤率。S.菌和大肠缺乏细胞抗氧化剂谷胱甘肽的大肠杆菌菌株与含有谷胱甘肽的菌株对单线态氧的毒性作用的反应没有差异。然而，包含类胡萝卜素的八叠球菌和金黄色葡萄球菌菌株对单氧致死的抗性远远高于同一物种的无类胡萝卜素突变体和缺乏高水平保护性类胡萝卜素的其他革兰氏阳性物种。
Gram-negative and gram-positive bacteria were found to display different sensitivities to pure singlet oxygen generated outside of cells. Killing curves for Salmonella typhimurium and Escherichia coli strains were indicative of multihit killing, whereas curves for Sarcina lutea, Staphylococcus aureus, Streptococcus lactis, and Streptococcus faecalis exhibited single-hit kinetics. The S. typhimurium deep rough strain TA1975, which lacks nearly all of the cell wall lipopolysaccharide coat and manifests concomitant enhancement of penetration by some exogeneous substances, responded to single oxygen wit initially faster inactivation than did the S. typhimurium wild-type strain, although the maximum rates of killing appeared to be quite similar. The structure of the cell wall thus plays an important role in susceptibility to single oxygen. The outer membrane-lipopolysaccharide protion of the gram-negative cell wall initially protects the bacteria from extracellular singlet oxygen, although it may also serve as a source for secondary reaction products which accentuate the rates of cell killing. S. typhimurium and E. coli strains lacking the cellular antioxidant, glutathione, showed no difference from strains containing glutathione in response to the toxic effects of singlet oxygen. Strains of Sarcina lutea and Staphylococcus aureus that contained carotenoids, however, were far more resistant to single oxygen lethality than were both carotenoidless mutants of the same species and other gram-positive species lacking high levels of protective carotenoids.